Table 2. Deactivation rates for EQ, EQQ,... | Rockefeller University Press

Table 2.

Deactivation rates for EQ, EQQ, and EQQQQ before and after ML277 treatment

	Mean (s)	SEM	n	Mean (s)	SEM	n	P value
Voltage (mV)	EQ			EQ + ML277
−50	1.65	0.09	10	1.53	0.06	7	ns
−60	1.82	0.12	9	1.60	0.05	7	ns
−70	1.53	0.36	8	1.81	0.10	5	ns
−80	0.76	0.03	10	0.60	0.04	8	ns
−90	0.70	0.03	10	0.60	0.04	8	ns
−100	0.57	0.03	10	0.50	0.02	8	ns
	EQQ			EQQ + ML277
−50	1.39	0.20	7	2.55	0.16	5	P = 0.0004
−60	1.08	0.14	7	2.42	0.15	5	P < 0.0001
−70	0.74	0.10	6	2.62	0.52	5	P < 0.0001
−80	0.54	0.10	5	1.30	0.12	4	ns
−90	0.43	0.07	6	1.08	0.10	5	ns
−100	0.47	0.06	3	0.98	0.16	3	ns
	EQQQQ			EQQQQ + ML277
−50	1.02	0.14	5	2.18	0.26	4	P = 0.0008
−60	0.78	0.15	5	2.52	0.53	3	P < 0.0001
−70	0.69	0.08	5	2.26	0.29	5	P < 0.0001
−80	0.40	0.10	5	1.35	0.23	5	P = 0.0071
−90	0.38	0.11	6	1.35	0.22	5	P = 0.0016
−100	0.26	0.01	3	0.90	0.08	3	ns

	Mean (s)	SEM	n	Mean (s)	SEM	n	P value
Voltage (mV)	EQ			EQ + ML277
−50	1.65	0.09	10	1.53	0.06	7	ns
−60	1.82	0.12	9	1.60	0.05	7	ns
−70	1.53	0.36	8	1.81	0.10	5	ns
−80	0.76	0.03	10	0.60	0.04	8	ns
−90	0.70	0.03	10	0.60	0.04	8	ns
−100	0.57	0.03	10	0.50	0.02	8	ns
	EQQ			EQQ + ML277
−50	1.39	0.20	7	2.55	0.16	5	P = 0.0004
−60	1.08	0.14	7	2.42	0.15	5	P < 0.0001
−70	0.74	0.10	6	2.62	0.52	5	P < 0.0001
−80	0.54	0.10	5	1.30	0.12	4	ns
−90	0.43	0.07	6	1.08	0.10	5	ns
−100	0.47	0.06	3	0.98	0.16	3	ns
	EQQQQ			EQQQQ + ML277
−50	1.02	0.14	5	2.18	0.26	4	P = 0.0008
−60	0.78	0.15	5	2.52	0.53	3	P < 0.0001
−70	0.69	0.08	5	2.26	0.29	5	P < 0.0001
−80	0.40	0.10	5	1.35	0.23	5	P = 0.0071
−90	0.38	0.11	6	1.35	0.22	5	P = 0.0016
−100	0.26	0.01	3	0.90	0.08	3	ns

To obtain tail currents for measurement of deactivation rates, cells were held at −80 mV, and pulsed to +60 mV for 4 s followed by a 10-s pulse to a range of potentials from −50 to −100 mV in 10-mV steps. Exponential fits and one-way ANOVA statistical comparison were performed using GraphPad Prism.

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