Table 1. V 1/2 of activation and slope... | Rockefeller University Press

Table 1.

V_1/2 of activation and slope of G-V plots

		Control			ML277		P value
Construct	V_1/2 (mV)	Slope	n	V_1/2 (mV)	Slope	n
EQ	30.1 ± 3.0	21.9 ± 1.0	12	17.5 ± 4.5	21.2 ± 1.5	10	0.003
EQQ	12.4 ± 2.3	19.0 ± 1.3	9	4.9 ± 2.9	18.5 ± 0.9	7	0.006
EQQQQ	−1.3 ± 2.2	18.0 ± 2.5	8	−12.3 ± 3.8	16.5 ± 2.0	6	0.031
Q	−20.9 ± 2.3	10.5 ± 2.0	4				-
Q*QQQ	−10.3 ± 4.8	18.6 ± 2.1	4				-
Q*Q	3.5 ± 2.2	15.6 ± 1.3	7	−0.5 ± 7.5	14.6 ± 3.8	3	ns
QQQQ	−13.3 ± 2.8	17.2 ± 2.1	4	−14.2 ± 1.5	13.5 ± 1.2	3	ns
QQQQ*	5.4 ± 4.6	13.5 ± 1.5	6	7.6 ± 3.0	15.9 ± 1.0	4	ns
K41C-E’QQQQ	−9.0 ± 7.4	16.3 ± 3.1	3				-
K41C-E’QQ	−7.0 ± 2.3	20.1 ± 1.7	6	−50.9 ± 10.9	45.8 ± 5.39	5	0.0003
KCNE5-Q	∼134	∼35	3	∼54	∼60	4

		Control			ML277		P value
Construct	V_1/2 (mV)	Slope	n	V_1/2 (mV)	Slope	n
EQ	30.1 ± 3.0	21.9 ± 1.0	12	17.5 ± 4.5	21.2 ± 1.5	10	0.003
EQQ	12.4 ± 2.3	19.0 ± 1.3	9	4.9 ± 2.9	18.5 ± 0.9	7	0.006
EQQQQ	−1.3 ± 2.2	18.0 ± 2.5	8	−12.3 ± 3.8	16.5 ± 2.0	6	0.031
Q	−20.9 ± 2.3	10.5 ± 2.0	4				-
Q*QQQ	−10.3 ± 4.8	18.6 ± 2.1	4				-
Q*Q	3.5 ± 2.2	15.6 ± 1.3	7	−0.5 ± 7.5	14.6 ± 3.8	3	ns
QQQQ	−13.3 ± 2.8	17.2 ± 2.1	4	−14.2 ± 1.5	13.5 ± 1.2	3	ns
QQQQ*	5.4 ± 4.6	13.5 ± 1.5	6	7.6 ± 3.0	15.9 ± 1.0	4	ns
K41C-E’QQQQ	−9.0 ± 7.4	16.3 ± 3.1	3				-
K41C-E’QQ	−7.0 ± 2.3	20.1 ± 1.7	6	−50.9 ± 10.9	45.8 ± 5.39	5	0.0003
KCNE5-Q	∼134	∼35	3	∼54	∼60	4

V_1/2 of activation was obtained from −40 mV tail portion of voltage-clamp protocols (−90 mV holding potential, pulsed from −90 up to +60 to +100 mV in 10 mV steps for 4 s, then to −40 mV for 1 s). Statistical comparison was performed using unpaired t tests in GraphPad Prism.

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