Table 2. smFRET conformational states... | Rockefeller University Press

Table 2.

smFRET conformational states occupied by AMPA receptor

Construct	State	Occupancy (%)	StaSI efficiency state	FRET distance (Å)	Gaussian state	Cα–Cα structure distance (Å)
Construct	State	Occupancy (%)	StaSI efficiency state	FRET distance (Å)	Gaussian state	(Protein Data Bank accession no.)
AMPA receptor (D23C) apo	State 1	94.55	0.88	37 ± 0.5	0.88 ± 0.01	35
	State 1	94.55	0.88	37 ± 0.5	0.88 ± 0.01	(4U2P^a)
	State 2	5.45	0.55	50 ± 0.7	0.53 ± 0.02	41
	State 2	5.45	0.55	50 ± 0.7	0.53 ± 0.02	(5L1B^a)
AMPA receptor (D23C)/γ8 apo	State 1	87.72	0.89	34 ± 0.8	0.92 ± 0.01
AMPA receptor (D23C)/γ8 apo	State 2	12.28	0.61	46 ± 0.7	0.64 ± 0.02
AMPA receptor (D23C) Glu + CTZ^b	State 1	69.18	0.89	35 ± 0.7	0.90 ± 0.01	41
						(4UQK^c)
						43
	State 2	30.82	0.76	42 ± 0.8	0.76 ± 0.02	(6DLZ^c)
						44
						(5WEO^c)
AMPA receptor (D23C) Glu^b	State 1	17.0	0.90	34 ± 2.3	0.92 ± 0.03	33
	State 2	35.8	0.75	42 ± 0.4	0.76 ± 0.01	(4U1Y^d)
	State 3	44.4	0.60	48 ± 0.3	0.60 ± 0.01	41
	State 4	2.1	0.44	52 ± 0.7	0.46 ± 0.02	(5VHZ^d)
AMPA receptor (D23C)/γ8 Glu	State 1	54.95	0.91	32 ± 1.0	0.94 ± 0.01
AMPA receptor (D23C)/γ8 Glu	State 2	45.05	0.70	42 ± 1.1	0.76 ± 0.03
AMPA receptor (L467C) apo	State 1	74.41	0.90	34 ± 0.8	0.92 ± 0.01	33
	State 1	74.41	0.90	34 ± 0.8	0.92 ± 0.01	(4U2P^a)
	State 2	25.59	0.68	45 ± 0.7	0.68 ± 0.02	33
	State 2	25.59	0.68	45 ± 0.7	0.68 ± 0.02	(5L1B^a)
AMPA receptor (L467C)/γ8 apo	State 1	65.74	0.92	34 ± 0.8	0.92 ± 0.01
AMPA receptor (L467C)/γ8 apo	State 2	34.26	0.72	44 ± 0.7	0.72 ± 0.02
AMPA receptor (L467C) Glu + CTZ	State 1	40.60	0.94	31 ± 2.1	0.95 ± 0.02	33
	State 1	40.60	0.94	31 ± 2.1	0.95 ± 0.02	(4UQK^c)
	State 2	17.91	0.87	38 ± 1.0	0.86 ± 0.02	34
	State 2	17.91	0.87	38 ± 1.0	0.86 ± 0.02	(6DLZ^c)
	State 3	24.34	0.77	42 ± 1.9	0.75 ± 0.05	34
	State 4	17.15	0.61	47 ± 0.3	0.61 ± 0.1	(5WEO^c)
AMPA receptor (L467C)/γ8 Glu	State 1	35.89	0.94	30 ± 2.6	0.96 ± 0.02
	State 2	29.68	0.83	39 ± 2.4	0.84 ± 0.05
	State 3	17.34	0.70	44 ± 2.5	0.70 ± 0.07
	State 4	17.09	0.55	50 ± 1.3	0.52 ± 0.04
AMPA receptor (L467C) Glu	State 1	27.32	0.89	35 ± 1.4	0.91 ± 0.02	33
	State 2	52.32	0.76	42 ± 0.8	0.77 ± 0.02	(4U1Y^d)
	State 3	16.64	0.60	48 ± 3.3	0.58 ± 0.1	40
	State 4	3.72	0.46	53 ± 3.6	0.42 ± 0.1	(5VHZ^d)

Construct	State	Occupancy (%)	StaSI efficiency state	FRET distance (Å)	Gaussian state	Cα–Cα structure distance (Å)
Construct	State	Occupancy (%)	StaSI efficiency state	FRET distance (Å)	Gaussian state	(Protein Data Bank accession no.)
AMPA receptor (D23C) apo	State 1	94.55	0.88	37 ± 0.5	0.88 ± 0.01	35
	State 1	94.55	0.88	37 ± 0.5	0.88 ± 0.01	(4U2Pa)
	State 2	5.45	0.55	50 ± 0.7	0.53 ± 0.02	41
	State 2	5.45	0.55	50 ± 0.7	0.53 ± 0.02	(5L1Ba)
AMPA receptor (D23C)/γ8 apo	State 1	87.72	0.89	34 ± 0.8	0.92 ± 0.01
AMPA receptor (D23C)/γ8 apo	State 2	12.28	0.61	46 ± 0.7	0.64 ± 0.02
AMPA receptor (D23C) Glu + CTZb	State 1	69.18	0.89	35 ± 0.7	0.90 ± 0.01	41
						(4UQKc)
						43
	State 2	30.82	0.76	42 ± 0.8	0.76 ± 0.02	(6DLZc)
						44
						(5WEOc)
AMPA receptor (D23C) Glub	State 1	17.0	0.90	34 ± 2.3	0.92 ± 0.03	33
	State 2	35.8	0.75	42 ± 0.4	0.76 ± 0.01	(4U1Yd)
	State 3	44.4	0.60	48 ± 0.3	0.60 ± 0.01	41
	State 4	2.1	0.44	52 ± 0.7	0.46 ± 0.02	(5VHZd)
AMPA receptor (D23C)/γ8 Glu	State 1	54.95	0.91	32 ± 1.0	0.94 ± 0.01
AMPA receptor (D23C)/γ8 Glu	State 2	45.05	0.70	42 ± 1.1	0.76 ± 0.03
AMPA receptor (L467C) apo	State 1	74.41	0.90	34 ± 0.8	0.92 ± 0.01	33
	State 1	74.41	0.90	34 ± 0.8	0.92 ± 0.01	(4U2Pa)
	State 2	25.59	0.68	45 ± 0.7	0.68 ± 0.02	33
	State 2	25.59	0.68	45 ± 0.7	0.68 ± 0.02	(5L1Ba)
AMPA receptor (L467C)/γ8 apo	State 1	65.74	0.92	34 ± 0.8	0.92 ± 0.01
AMPA receptor (L467C)/γ8 apo	State 2	34.26	0.72	44 ± 0.7	0.72 ± 0.02
AMPA receptor (L467C) Glu + CTZ	State 1	40.60	0.94	31 ± 2.1	0.95 ± 0.02	33
	State 1	40.60	0.94	31 ± 2.1	0.95 ± 0.02	(4UQKc)
	State 2	17.91	0.87	38 ± 1.0	0.86 ± 0.02	34
	State 2	17.91	0.87	38 ± 1.0	0.86 ± 0.02	(6DLZc)
	State 3	24.34	0.77	42 ± 1.9	0.75 ± 0.05	34
	State 4	17.15	0.61	47 ± 0.3	0.61 ± 0.1	(5WEOc)
AMPA receptor (L467C)/γ8 Glu	State 1	35.89	0.94	30 ± 2.6	0.96 ± 0.02
	State 2	29.68	0.83	39 ± 2.4	0.84 ± 0.05
	State 3	17.34	0.70	44 ± 2.5	0.70 ± 0.07
	State 4	17.09	0.55	50 ± 1.3	0.52 ± 0.04
AMPA receptor (L467C) Glu	State 1	27.32	0.89	35 ± 1.4	0.91 ± 0.02	33
	State 2	52.32	0.76	42 ± 0.8	0.77 ± 0.02	(4U1Yd)
	State 3	16.64	0.60	48 ± 3.3	0.58 ± 0.1	40
	State 4	3.72	0.46	53 ± 3.6	0.42 ± 0.1	(5VHZd)

STaSI, step transition and state identification used to determine the states as stated in Materials and methods. Gaussian states were obtained from fitting the observed data.

Structures 4U2P and 5L1B represent the apo structure of the receptor.

Data from Shaikh et al. (2016).

Structures 4UQK, 6DLZ, and 5WEO represent the open structure of the receptor.

Structures 4U1Y and 5VHZ represent the desensitized structure of the receptor.

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