Table 2. Kinetics of... | Rockefeller University Press

Table 2.

Kinetics of depolarization-triggered [Ca²⁺]_i transients in δ-cells

[cAMP]_i (μM)	Treatment	[Ca²⁺]_peak (nM)	Half width (ms)	n
0	n/a	185 ± 60	226 ± 30	8
1	n/a	347 ± 88	207 ± 17	5
10	n/a	326 ± 35	282 ± 17	3
100	n/a	353 ± 56^{^a}	347 ± 36^{^a}	5
0	100 µM 2′-O-Me-cAMP	232 ± 34^{^b}	465 ± 121^{^a}	7
100	10 µM thapsigargin	286 ± 45	301 ± 12	4
100	1 µM myr-PKI	347 ± 53^{^a}	558 ± 106^{^a}	6
100	25 µM ESI-05	225 ± 39	291 ± 34	4

[cAMP]_i (μM)	Treatment	[Ca²⁺]_peak (nM)	Half width (ms)	n
0	n/a	185 ± 60	226 ± 30	8
1	n/a	347 ± 88	207 ± 17	5
10	n/a	326 ± 35	282 ± 17	3
100	n/a	353 ± 56^{^a}	347 ± 36^{^a}	5
0	100 µM 2′-O-Me-cAMP	232 ± 34^{^b}	465 ± 121^{^a}	7
100	10 µM thapsigargin	286 ± 45	301 ± 12	4
100	1 µM myr-PKI	347 ± 53^{^a}	558 ± 106^{^a}	6
100	25 µM ESI-05	225 ± 39	291 ± 34	4

Peak ([Ca²⁺]_peak) and duration (half width) of [Ca²⁺]_i transients triggered by 300-ms depolarizations from −70 mV to 0 mV in δ-cells infused with intracellular solutions containing cAMP with concentrations indicated. The Treatment column lists the compounds that were infused into δ-cells at the indicated concentrations in the presence of 100 µM cAMP (with the exception of thapsigargin, which was applied extracellularly during the pretreatment of the islets). Data are presented as mean values ± SEM of indicated number of independent experiments (n) from two to eight animals. All the measurements were made using standard whole-cell technique. n/a, not applicable.

P < 0.05 vs. control (no addition of cAMP).

P < 0.05 vs. 100 µM cAMP alone.

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