Activation pH0.5 values and Hill slopes for ASIC3 in the presence of PUFAs and PUFA derivatives
| Tail . | Head group . | pH0.5 . | SEM . | Hill . | SEM . | n . | P value . |
|---|---|---|---|---|---|---|---|
| Control | 6.56 | 0.01 | 2.8 | 0.07 | 70 | ||
| 18(9) | Carboxyl | 6.57 | 0.04 | 2.7 | 0.13 | 7 | 1 |
| 18(6,9,12) | Carboxyl | 6.59 | 0.01 | 2.8 | 0.11 | 11 | 1 |
| 18(6,9,12,15) | Carboxyl | 6.62 | 0.04 | 3.0 | 0.22 | 6 | 0.869 |
| 18(9,12) | Carboxyl | 6.64 | 0.03 | 2.5 | 0.08 | 6 | 0.508 |
| 18(9,12,15) | Carboxyl | 6.65 | 0.02 | 3.1 | 0.22 | 6 | 0.193 |
| 19(4,7,10,13,16) | Carboxyl | 6.57 | 0.02 | 2.6 | 0.10 | 6 | 1 |
| 20(14) | Carboxyl | 6.49 | 0.02 | 2.5 | 0.05 | 6 | 0.424 |
| 20(11,14) | Carboxyl | 6.49 | 0.01 | 2.7 | 0.12 | 4 | 0.862 |
| 20(8,11,14) | Carboxyl | 6.60 | 0.01 | 2.9 | 0.13 | 5 | 1 |
| 20(5,8,11,14,17) | Carboxyl | 6.62 | 0.02 | 2.8 | 0.17 | 11 | 0.618 |
| 20(5,8,11,14) | Carboxyl | 6.68 | 0.01 | 2.9 | 0.09 | 7 | 0.005 |
| 20(5,8,11) | Carboxyl | 6.85 | 0.04 | 3.5 | 0.27 | 8 | <0.001 |
| 20(5,11,14) | Carboxyl | 6.85 | 0.02 | 2.9 | 0.56 | 4 | <0.001 |
| 20(5,8,14) | Carboxyl | 6.86 | 0.04 | 3.1 | 0.16 | 5 | <0.001 |
| 20(11,14,17) | Carboxyl | 6.86 | 0.03 | 3.6 | 0.30 | 7 | <0.001 |
| 20(8,11,14,17) | Carboxyl | 6.88 | 0.03 | 3.2 | 0.23 | 9 | <0.001 |
| 22(13) | Carboxyl | 6.54 | 0.02 | 2.6 | 0.04 | 6 | 1 |
| 22(7,10,13,16) | Carboxyl | 6.55 | 0.02 | 2.5 | 0.19 | 6 | 1 |
| 22(13,16,19) | Carboxyl | 6.60 | 0.02 | 2.6 | 0.19 | 4 | 1 |
| 22(13,16) | Carboxyl | 6.58 | 0.02 | 2.8 | 0.09 | 6 | 1 |
| 22(7,10,13,16,19) | Carboxyl | 6.64 | 0.03 | 3.1 | 0.15 | 6 | 0.369 |
| 22(4,10,13,16) | Carboxyl | 6.83 | 0.03 | 3.5 | 0.21 | 5 | <0.001 |
| 22(4,7,10,13,16,19) | Carboxyl | 6.83 | 0.03 | 3.2 | 0.18 | 5 | <0.001 |
| 22(4,7,10,13,16) | Carboxyl | 6.84 | 0.03 | 2.7 | 0.26 | 4 | <0.001 |
| 20(5,8,11,14) | Methyl amide | 6.54 | 0.05 | 2.6 | 0.12 | 5 | 1 |
| 20(5,8,11,14) | Ethyl ester | 6.59 | 0.01 | 2.6 | 0.12 | 4 | 1 |
| 20(5,8,11,14) | Ethanolamide | 6.62 | 0.04 | 2.6 | 0.15 | 5 | 0.912 |
| 20(5,8,11,14) | Methyl ester | 6.64 | 0.03 | 2.9 | 0.05 | 5 | 0.648 |
| 20(5,8,11,14) | Alanine | 6.75 | 0.02 | 3.2 | 0.23 | 4 | <0.001 |
| 20(5,8,11,14) | Serine | 6.86 | 0.03 | 3.8 | 0.20 | 7 | <0.001 |
| 20(5,8,11,14) | Glycine | 6.90 | 0.03 | 3.5 | 0.21 | 4 | <0.001 |
| 20(5,8,11,14) | Ethanolamide phosphate | 6.67 | 0.03 | 2.7 | 0.20 | 6 | 0.022 |
| 20(5,8,11,14) | Taurine | 6.52 | 0.01 | 2.6 | 0.02 | 4 | 1 |
| 18(9) | Glycine | 6.72 | 0.02 | 3.3 | 0.13 | 5 | <0.001 |
| 18(9,12) | Glycine | 6.89 | 0.02 | 3.3 | 0.39 | 5 | <0.001 |
| 22(4,10,13,16) | Methyl ester | 6.64 | 0.06 | 3.0 | 0.38 | 5 | 0.648 |
| Glycine (100 µM) | 6.52 | 0.01 | 3 | 1 | |||
| Internal AG (0 min) | 6.58 | 0.07 | 3 | 1 | |||
| Internal AG (5 min) | 6.54 | 0.05 | 3 | 1 | |||
| Internal AG (10 min) | 6.57 | 0.04 | 3 | 1 |
| Tail . | Head group . | pH0.5 . | SEM . | Hill . | SEM . | n . | P value . |
|---|---|---|---|---|---|---|---|
| Control | 6.56 | 0.01 | 2.8 | 0.07 | 70 | ||
| 18(9) | Carboxyl | 6.57 | 0.04 | 2.7 | 0.13 | 7 | 1 |
| 18(6,9,12) | Carboxyl | 6.59 | 0.01 | 2.8 | 0.11 | 11 | 1 |
| 18(6,9,12,15) | Carboxyl | 6.62 | 0.04 | 3.0 | 0.22 | 6 | 0.869 |
| 18(9,12) | Carboxyl | 6.64 | 0.03 | 2.5 | 0.08 | 6 | 0.508 |
| 18(9,12,15) | Carboxyl | 6.65 | 0.02 | 3.1 | 0.22 | 6 | 0.193 |
| 19(4,7,10,13,16) | Carboxyl | 6.57 | 0.02 | 2.6 | 0.10 | 6 | 1 |
| 20(14) | Carboxyl | 6.49 | 0.02 | 2.5 | 0.05 | 6 | 0.424 |
| 20(11,14) | Carboxyl | 6.49 | 0.01 | 2.7 | 0.12 | 4 | 0.862 |
| 20(8,11,14) | Carboxyl | 6.60 | 0.01 | 2.9 | 0.13 | 5 | 1 |
| 20(5,8,11,14,17) | Carboxyl | 6.62 | 0.02 | 2.8 | 0.17 | 11 | 0.618 |
| 20(5,8,11,14) | Carboxyl | 6.68 | 0.01 | 2.9 | 0.09 | 7 | 0.005 |
| 20(5,8,11) | Carboxyl | 6.85 | 0.04 | 3.5 | 0.27 | 8 | <0.001 |
| 20(5,11,14) | Carboxyl | 6.85 | 0.02 | 2.9 | 0.56 | 4 | <0.001 |
| 20(5,8,14) | Carboxyl | 6.86 | 0.04 | 3.1 | 0.16 | 5 | <0.001 |
| 20(11,14,17) | Carboxyl | 6.86 | 0.03 | 3.6 | 0.30 | 7 | <0.001 |
| 20(8,11,14,17) | Carboxyl | 6.88 | 0.03 | 3.2 | 0.23 | 9 | <0.001 |
| 22(13) | Carboxyl | 6.54 | 0.02 | 2.6 | 0.04 | 6 | 1 |
| 22(7,10,13,16) | Carboxyl | 6.55 | 0.02 | 2.5 | 0.19 | 6 | 1 |
| 22(13,16,19) | Carboxyl | 6.60 | 0.02 | 2.6 | 0.19 | 4 | 1 |
| 22(13,16) | Carboxyl | 6.58 | 0.02 | 2.8 | 0.09 | 6 | 1 |
| 22(7,10,13,16,19) | Carboxyl | 6.64 | 0.03 | 3.1 | 0.15 | 6 | 0.369 |
| 22(4,10,13,16) | Carboxyl | 6.83 | 0.03 | 3.5 | 0.21 | 5 | <0.001 |
| 22(4,7,10,13,16,19) | Carboxyl | 6.83 | 0.03 | 3.2 | 0.18 | 5 | <0.001 |
| 22(4,7,10,13,16) | Carboxyl | 6.84 | 0.03 | 2.7 | 0.26 | 4 | <0.001 |
| 20(5,8,11,14) | Methyl amide | 6.54 | 0.05 | 2.6 | 0.12 | 5 | 1 |
| 20(5,8,11,14) | Ethyl ester | 6.59 | 0.01 | 2.6 | 0.12 | 4 | 1 |
| 20(5,8,11,14) | Ethanolamide | 6.62 | 0.04 | 2.6 | 0.15 | 5 | 0.912 |
| 20(5,8,11,14) | Methyl ester | 6.64 | 0.03 | 2.9 | 0.05 | 5 | 0.648 |
| 20(5,8,11,14) | Alanine | 6.75 | 0.02 | 3.2 | 0.23 | 4 | <0.001 |
| 20(5,8,11,14) | Serine | 6.86 | 0.03 | 3.8 | 0.20 | 7 | <0.001 |
| 20(5,8,11,14) | Glycine | 6.90 | 0.03 | 3.5 | 0.21 | 4 | <0.001 |
| 20(5,8,11,14) | Ethanolamide phosphate | 6.67 | 0.03 | 2.7 | 0.20 | 6 | 0.022 |
| 20(5,8,11,14) | Taurine | 6.52 | 0.01 | 2.6 | 0.02 | 4 | 1 |
| 18(9) | Glycine | 6.72 | 0.02 | 3.3 | 0.13 | 5 | <0.001 |
| 18(9,12) | Glycine | 6.89 | 0.02 | 3.3 | 0.39 | 5 | <0.001 |
| 22(4,10,13,16) | Methyl ester | 6.64 | 0.06 | 3.0 | 0.38 | 5 | 0.648 |
| Glycine (100 µM) | 6.52 | 0.01 | 3 | 1 | |||
| Internal AG (0 min) | 6.58 | 0.07 | 3 | 1 | |||
| Internal AG (5 min) | 6.54 | 0.05 | 3 | 1 | |||
| Internal AG (10 min) | 6.57 | 0.04 | 3 | 1 |
All values are measured at 10 µM external application of lipid unless otherwise indicated. n denotes number of individual cells. P values were calculated with a post hoc Dunnett’s test comparing each pH0.5 dataset to the control dataset.