Table 1. Thermal denaturation MelB St... | Rockefeller University Press

Table 1.

Thermal denaturation

MelB_St	Buffer^a	Number of tests	10% unfolding (°C)	90% unfolding (°C)	50% unfolding (Tm, °C)	ΔTm (°C; P value)
WT	Apo	3	46.13 ± 0.70^b	62.26 ± 0.50	53.29^c ± 0.05	/
	K⁺	2	46.06 ± 0.02	59.94 ± 0.69	53.34 ± 0.04	0.05^d (>0.05^e)
	Na⁺	3	49.07 ± 0.28	60.06 ± 0.29	54.83 ± 0.03	1.54 (<0.05)
	Mel	2	45.95 ± 0.47	64.24 ± 0.42	54.07 ± 0.07	0.78 (<0.05)
	Mel and Na⁺	2	49.42 ± 0.13	61.76 ± 0.09	56.71 ± 0.05	3.43 (<0.05)
D55C	Apo	2			>80^f
	K⁺	2	46.51 ± 0.71	65.07 ± 0.06	56.15 ± 0.16	>14
	Na⁺	3	46.35 ± 0.25	66.51 ± 0.31	56.75 ± 0.25	>14
	Mel	2			>80
	Mel and Na⁺	2	46.25 ± 0.20	68.52 ± 0.27	58.15 ± 0.11	1.40 (<0.05)

MelB_St	Buffera	Number of tests	10% unfolding (°C)	90% unfolding (°C)	50% unfolding (Tm, °C)	ΔTm (°C; P value)
WT	Apo	3	46.13 ± 0.70b	62.26 ± 0.50	53.29c ± 0.05	/
	K⁺	2	46.06 ± 0.02	59.94 ± 0.69	53.34 ± 0.04	0.05d (>0.05e)
	Na⁺	3	49.07 ± 0.28	60.06 ± 0.29	54.83 ± 0.03	1.54 (<0.05)
	Mel	2	45.95 ± 0.47	64.24 ± 0.42	54.07 ± 0.07	0.78 (<0.05)
	Mel and Na⁺	2	49.42 ± 0.13	61.76 ± 0.09	56.71 ± 0.05	3.43 (<0.05)
D55C	Apo	2			>80f
	K⁺	2	46.51 ± 0.71	65.07 ± 0.06	56.15 ± 0.16	>14
	Na⁺	3	46.35 ± 0.25	66.51 ± 0.31	56.75 ± 0.25	>14
	Mel	2			>80
	Mel and Na⁺	2	46.25 ± 0.20	68.52 ± 0.27	58.15 ± 0.11	1.40 (<0.05)

CD assay buffers consisted of 10 mM KPi, pH 7.5, 10% glycerol, and 0.035% UDM with a given component at 50 mM of each.

SEM.

T_m value at which 50% protein unfolded.

The difference in T_m value when compared with apo (in WT) or the buffer containing Na⁺ (in D55C mutant).

Unpaired t test; P < 0.05 was considered statistically significant.

Completion of denaturation cannot be approached, so this value is an estimation based on the unfolded fraction.

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