Table 1. Primers for amplification of... | Rockefeller University Press

Table 1.

Primers for amplification of 5′- and 3′-arm and for screening

Primer	Sequence^a	Location
Cloning
5′-arm forward	AACTCGAGGATATCAAGATGATGGTGCCCATTAC	34739922–34739944^b
5′-arm reverse	GAGAATTCTCCATAAATATGACAGGAAGAGTAG	34740097–34740121^b
3′-arm forward	AAGGATCCCTTCTCTACCACTTAGAACCAAG	34774307–34774329^b
3′-arm reverse	ATCATACCGCGGATATCAACTTATGACCGTACCAACTG	34774490–34774510^b
Southern
Cald1 forward	CTACCACAAACCTTACTGCTGAGC	69123–69100^c
Cald1 reverse	GGTGCTGGCCTCATTGAAAA	68582–68601^c
COL12A1 forward	GATGGTACTTACAGATATATGG	Collagen, type XII α1
COL12A1 forward	GATGGTACTTACAGATATATGG	126865–126844^c
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	Collagen, type XII α1
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	126242–126262^c
Genotyping
Cald1 forward	CATCAAGTCCTACCAGAAGAA	80083–80063^d
Cald1 reverse	CCCTTTCATTCACTCCTTTCC	79392–79412^d
neomycin	CATCGCATTGTCTGAGTAGG	Neomycin
Cald1 reverse	TTTGTACAATGACGCTGTATGC	51269–51290^d

Primer	Sequencea	Location
Cloning
5′-arm forward	AACTCGAGGATATCAAGATGATGGTGCCCATTAC	34739922–34739944b
5′-arm reverse	GAGAATTCTCCATAAATATGACAGGAAGAGTAG	34740097–34740121b
3′-arm forward	AAGGATCCCTTCTCTACCACTTAGAACCAAG	34774307–34774329b
3′-arm reverse	ATCATACCGCGGATATCAACTTATGACCGTACCAACTG	34774490–34774510b
Southern
Cald1 forward	CTACCACAAACCTTACTGCTGAGC	69123–69100c
Cald1 reverse	GGTGCTGGCCTCATTGAAAA	68582–68601c
COL12A1 forward	GATGGTACTTACAGATATATGG	Collagen, type XII α1
COL12A1 forward	GATGGTACTTACAGATATATGG	126865–126844c
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	Collagen, type XII α1
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	126242–126262c
Genotyping
Cald1 forward	CATCAAGTCCTACCAGAAGAA	80083–80063d
Cald1 reverse	CCCTTTCATTCACTCCTTTCC	79392–79412d
neomycin	CATCGCATTGTCTGAGTAGG	Neomycin
Cald1 reverse	TTTGTACAATGACGCTGTATGC	51269–51290d

Gene-specific sequence is underlined.

CaD 1, GeneID accession number 109624; chromosome 6, NC_000072.6.

Mus musculus BAC clone RP24-141A7 from chromosome 9, GenBank accession number AC157477.2.

Mus musculus 6 BAC RP23-104H13, complete sequence.

Sharing Unavailable