Table 1. Primers for amplification of... | Rockefeller University Press

Table 1.

Primers for amplification of 5′- and 3′-arm and for screening

Primer	Sequence^a	Location
Cloning
5′-arm forward	AACTCGAGGATATCAAGATGATGGTGCCCATTAC	34739922–34739944^b
5′-arm reverse	GAGAATTCTCCATAAATATGACAGGAAGAGTAG	34740097–34740121^b
3′-arm forward	AAGGATCCCTTCTCTACCACTTAGAACCAAG	34774307–34774329^b
3′-arm reverse	ATCATACCGCGGATATCAACTTATGACCGTACCAACTG	34774490–34774510^b
Southern
Cald1 forward	CTACCACAAACCTTACTGCTGAGC	69123–69100^c
Cald1 reverse	GGTGCTGGCCTCATTGAAAA	68582–68601^c
COL12A1 forward	GATGGTACTTACAGATATATGG	Collagen, type XII α1
COL12A1 forward	GATGGTACTTACAGATATATGG	126865–126844^c
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	Collagen, type XII α1
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	126242–126262^c
Genotyping
Cald1 forward	CATCAAGTCCTACCAGAAGAA	80083–80063^d
Cald1 reverse	CCCTTTCATTCACTCCTTTCC	79392–79412^d
neomycin	CATCGCATTGTCTGAGTAGG	Neomycin
Cald1 reverse	TTTGTACAATGACGCTGTATGC	51269–51290^d

Primer	Sequence^a	Location
Cloning
5′-arm forward	AACTCGAGGATATCAAGATGATGGTGCCCATTAC	34739922–34739944^b
5′-arm reverse	GAGAATTCTCCATAAATATGACAGGAAGAGTAG	34740097–34740121^b
3′-arm forward	AAGGATCCCTTCTCTACCACTTAGAACCAAG	34774307–34774329^b
3′-arm reverse	ATCATACCGCGGATATCAACTTATGACCGTACCAACTG	34774490–34774510^b
Southern
Cald1 forward	CTACCACAAACCTTACTGCTGAGC	69123–69100^c
Cald1 reverse	GGTGCTGGCCTCATTGAAAA	68582–68601^c
COL12A1 forward	GATGGTACTTACAGATATATGG	Collagen, type XII α1
COL12A1 forward	GATGGTACTTACAGATATATGG	126865–126844^c
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	Collagen, type XII α1
COL12A1 reverse	GCAAAGAGGAGCAGGAAAAGG	126242–126262^c
Genotyping
Cald1 forward	CATCAAGTCCTACCAGAAGAA	80083–80063^d
Cald1 reverse	CCCTTTCATTCACTCCTTTCC	79392–79412^d
neomycin	CATCGCATTGTCTGAGTAGG	Neomycin
Cald1 reverse	TTTGTACAATGACGCTGTATGC	51269–51290^d

Gene-specific sequence is underlined.

CaD 1, GeneID accession number 109624; chromosome 6, NC_000072.6.

Mus musculus BAC clone RP24-141A7 from chromosome 9, GenBank accession number AC157477.2.

Mus musculus 6 BAC RP23-104H13, complete sequence.