Table 3. Hill fit parameters for Cd 2... | Rockefeller University Press

Table 3.

Hill fit parameters for Cd²⁺ affinity experiments

Channel	KAT1 WT
Equation^a, ^b	$Δ V_{50} = S t a r t + (E n d - S t a r t) \{\frac{{[C d^{2 +}]}^{H}}{K d^{H} + {[C d^{2 +}]}^{H}}\}$
pH^c	K_d^d	H^e	Start	End	Span^f
9	17.3 µM	7.4	−15.4	180.1	195.5
8	43.5 µM	3.0	−5.5	183.9	189.4
7	115 µM	2.2	−3.5	166.9	170.4
6	6.36 mM	0.9	−8.9	206.6	215.5

Channel	KAT1 WT
Equation^a, ^b	$Δ V_{50} = S t a r t + (E n d - S t a r t) \{\frac{{[C d^{2 +}]}^{H}}{K d^{H} + {[C d^{2 +}]}^{H}}\}$
pH^c	K_d^d	H^e	Start	End	Span^f
9	17.3 µM	7.4	−15.4	180.1	195.5
8	43.5 µM	3.0	−5.5	183.9	189.4
7	115 µM	2.2	−3.5	166.9	170.4
6	6.36 mM	0.9	−8.9	206.6	215.5

ΔV₅₀ is shift in V₅₀ (V₅₀[Cd²⁺] − V₅₀[control]) in mV.

[Cd²⁺] is concentration of external Cd²⁺ in M.

Recorded in 20 mM external K⁺. Solutions were buffered with 5 mM HEPES, and pH was calibrated before recording session.

Midpoint [Cd²⁺].

Hill slope.

Maximal predicted ΔV₅₀ in mV.