Table 2. Boltzmann fit parameters for pH... | Rockefeller University Press

Table 2.

Boltzmann fit parameters for pH experiments

Channel	pH^a	V₅₀^b	s^c	n^d
WT	9	−101.0 ± 2.2	25.3 ± 1.3	8
	8	−97.4 ± 1.0	21.6 ± 0.5	8
	7	−97.7 ± 1.3	22.0 ± 0.4	8
	6	−97.1 ± 1.7	21.1 ± 0.3	8
	5	−91.8 ± 2.6	21.0 ± 0.2	8
	4	−48.6 ± 1.5	18.3 ± 0.3	8
D95N	8	−3.1 ± 3.4	18.4 ± 0.9	8
	7	−3.1 ± 2.8	16.8 ± 0.8	8
	6	−1.8 ± 2.5	16.6 ± 0.6	8
	5	−2.4 ± 1.9	19.5 ± 0.7	8
	4	9.7 ± 2.5	16.9 ± 0.7	8
N99D	8	−115.7 ± 0.7	18.9 ± 0.2	8
	7	−113.0 ± 1.2	19.2 ± 0.1	8
	6	−106.6 ± 1.5	19.7 ± 0.1	8
	5	−98.7 ± 1.6	20.6 ± 0.3	8
	4	−65.5 ± 1.5	20.0 ± 0.8	8
Q149E	8	−81.2 ± 1.6	21.4 ± 0.5	8
	7	−77.6 ± 2.4	23.7 ± 0.9	8
	6	−67.8 ± 1.9	23.7 ± 1.2	8
	5	−52.5 ± 1.5	23.2 ± 1.0	8
	4	−15.6 ± 2.4	16.4 ± 0.8	8

Channel	pH^a	V₅₀^b	s^c	n^d
WT	9	−101.0 ± 2.2	25.3 ± 1.3	8
	8	−97.4 ± 1.0	21.6 ± 0.5	8
	7	−97.7 ± 1.3	22.0 ± 0.4	8
	6	−97.1 ± 1.7	21.1 ± 0.3	8
	5	−91.8 ± 2.6	21.0 ± 0.2	8
	4	−48.6 ± 1.5	18.3 ± 0.3	8
D95N	8	−3.1 ± 3.4	18.4 ± 0.9	8
	7	−3.1 ± 2.8	16.8 ± 0.8	8
	6	−1.8 ± 2.5	16.6 ± 0.6	8
	5	−2.4 ± 1.9	19.5 ± 0.7	8
	4	9.7 ± 2.5	16.9 ± 0.7	8
N99D	8	−115.7 ± 0.7	18.9 ± 0.2	8
	7	−113.0 ± 1.2	19.2 ± 0.1	8
	6	−106.6 ± 1.5	19.7 ± 0.1	8
	5	−98.7 ± 1.6	20.6 ± 0.3	8
	4	−65.5 ± 1.5	20.0 ± 0.8	8
Q149E	8	−81.2 ± 1.6	21.4 ± 0.5	8
	7	−77.6 ± 2.4	23.7 ± 0.9	8
	6	−67.8 ± 1.9	23.7 ± 1.2	8
	5	−52.5 ± 1.5	23.2 ± 1.0	8
	4	−15.6 ± 2.4	16.4 ± 0.8	8

Recorded in 20 mM external K⁺. Solutions for pH 9–6 were buffered with 5 mM HEPES, and solutions for pH 5–4 were buffered with 5 mM PIPES. Solution pH was calibrated before recording session.

Midpoint of single Boltzmann fit, mV (mean ± SEM).

Slope factor, mV (mean ± SEM).

Number of measurements.

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