Table 1.
Boltzmann fit parameters for Cd2+ experiments
Channel[Cd2+]aV50bscnd
WT −103.2 ± 1.8 22.3 ± 0.4 10 
300 46.5 ± 2.3 21.4 ± 0.3 10 
0 (pH 5) −95.2 ± 2.3 20.4 ± 0.2 
300 (pH 5) −106.1 ± 2.0 20.0 ± 0.2 
D95N 0.2 ± 2.8 11.6 ± 0.6 
300 −3.6 ± 1.5 13.6 ± 0.6 
N99D −111.8 ± 2.6 18.8 ± 0.5 
300 −80.3 ± 3.3 22.0 ± 1.1 
Q149E −77.1 ± 1.5 18.7 ± 0.4 
300 12.7 ± 1.9 18.0 ± 0.6 
Channel[Cd2+]aV50bscnd
WT −103.2 ± 1.8 22.3 ± 0.4 10 
300 46.5 ± 2.3 21.4 ± 0.3 10 
0 (pH 5) −95.2 ± 2.3 20.4 ± 0.2 
300 (pH 5) −106.1 ± 2.0 20.0 ± 0.2 
D95N 0.2 ± 2.8 11.6 ± 0.6 
300 −3.6 ± 1.5 13.6 ± 0.6 
N99D −111.8 ± 2.6 18.8 ± 0.5 
300 −80.3 ± 3.3 22.0 ± 1.1 
Q149E −77.1 ± 1.5 18.7 ± 0.4 
300 12.7 ± 1.9 18.0 ± 0.6 
a

External [Cd2+] in μM. Recorded in 20 mM external K+; pH 7.0 unless otherwise noted.

b

Midpoint of single Boltzmann fit, mV (mean ± SEM).

c

Slope factor, mV (mean ± SEM).

d

Number of measurements.

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