Table 1.
Comparison of features between human and lamprey orthologues, focusing on three major domains of function: channel behavior, regulation, and modulation
Lp-CFTRhCFTR
Functional domain: channel behavior
Open channel stability (open burst duration) Low High 
Frequency of subconductance states High Low 
Single-channel open conductance Low High 
Shape of I-V relationship Rectified Linear 
Sensitivity to (affinity for) ATP for channel opening Very low High 
Functional domain: regulation by phosphorylation 
Rate of activation by PKA-mediated phosphorylation Low High 
Number of predicted PKA sites in the R domain 
Functional domain: pharmacological modulation 
Effect of VX-770/ivacaftor (inhibition versus potentiation) Small inhibition Potentiation 
Inhibition by CFTRinh172 Low High 
Sensitivity to pore block by GlyH-101 None High 
Sensitivity to pore block by NPPB Low High 
Sensitivity to pore block by glibenclamide Equal Equal 
Lp-CFTRhCFTR
Functional domain: channel behavior
Open channel stability (open burst duration) Low High 
Frequency of subconductance states High Low 
Single-channel open conductance Low High 
Shape of I-V relationship Rectified Linear 
Sensitivity to (affinity for) ATP for channel opening Very low High 
Functional domain: regulation by phosphorylation 
Rate of activation by PKA-mediated phosphorylation Low High 
Number of predicted PKA sites in the R domain 
Functional domain: pharmacological modulation 
Effect of VX-770/ivacaftor (inhibition versus potentiation) Small inhibition Potentiation 
Inhibition by CFTRinh172 Low High 
Sensitivity to pore block by GlyH-101 None High 
Sensitivity to pore block by NPPB Low High 
Sensitivity to pore block by glibenclamide Equal Equal 

NPPB, 5-nitro-2-(3-phenyl-propylamino) benzoic acid. Related to Cui et al., 2019a.

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