Table 2.
Mean activation and deactivation time constants of HCN1 WT and NA/DC mutant in the presence and absence of TRIP8b (1a-4)
V/mVHCN1 WTHCN1 WT+ TRIP8bP valueHCN1 NA/DCHCN1 NA/DC + TRIP8bP value
−70 τ activation (ms) 540.5 ± 71.3 875.7 ± 135.3 4.1E−03 477.2 ± 33.1 476.2 ± 42.8 1.0 
−80 450.5 ± 74.4 721.1 ± 55.7 3.8E−03 386.6 ± 35.8 459.3 ± 46.7 0.4 
−90 319.4 ± 46.3 516.4 ± 41.0 2.3E−03 299.6 ± 28.5 363.5 ± 34.2 0.25 
−100 242.5 ± 28.9 364.0 ± 26.7 3.15E−03 238.8 ± 20.9 278.1 ± 21.0 0.3 
−110 185.4 ± 18.4 305.6 ± 28.0 7.5E−03 201.4 ± 14.9 220.4 ± 13.0 0.4 
−120 159.3 ± 13.2 257.6 ± 20.4 3.9E−03 177.9 ± 12.2 199.6 ± 21.6 0.4 
−130 141.2 ± 11.4 221.9 ± 21.9 6.4E−04 153.5 ± 9.9 152.7 ± 11.2 1.0 
−40 τ deactivation (ms) 117.7 ± 3.2 89.7 ± 6.2 7.7E−03 116.9 ± 8.7 120.8 ± 5.3 0.7 
V/mVHCN1 WTHCN1 WT+ TRIP8bP valueHCN1 NA/DCHCN1 NA/DC + TRIP8bP value
−70 τ activation (ms) 540.5 ± 71.3 875.7 ± 135.3 4.1E−03 477.2 ± 33.1 476.2 ± 42.8 1.0 
−80 450.5 ± 74.4 721.1 ± 55.7 3.8E−03 386.6 ± 35.8 459.3 ± 46.7 0.4 
−90 319.4 ± 46.3 516.4 ± 41.0 2.3E−03 299.6 ± 28.5 363.5 ± 34.2 0.25 
−100 242.5 ± 28.9 364.0 ± 26.7 3.15E−03 238.8 ± 20.9 278.1 ± 21.0 0.3 
−110 185.4 ± 18.4 305.6 ± 28.0 7.5E−03 201.4 ± 14.9 220.4 ± 13.0 0.4 
−120 159.3 ± 13.2 257.6 ± 20.4 3.9E−03 177.9 ± 12.2 199.6 ± 21.6 0.4 
−130 141.2 ± 11.4 221.9 ± 21.9 6.4E−04 153.5 ± 9.9 152.7 ± 11.2 1.0 
−40 τ deactivation (ms) 117.7 ± 3.2 89.7 ± 6.2 7.7E−03 116.9 ± 8.7 120.8 ± 5.3 0.7 

Data are reported as mean ± SEM. Statistical analysis performed with one-way ANOVA, followed by post-hoc Tukey test.

or Create an Account

Close Modal
Close Modal