Table 3. Orai-GCaMP6f fluorescence... | Rockefeller University Press

Table 3.

Orai-GCaMP6f fluorescence rise/fall kinetics

[Ca²⁺] mM	Pulse mV	Rise τ_Rise ms	95% CI	Fall τ_Fall ms	95% CI
20	−100	33	28–38	63	60–67
2	−100	71	62–80	62	58–65
0.6	−100	86	72–103	50	44–58
2	−100	81	69–95	59	53–66
2	−70	96	80–116	55	48–62
2	−40	91	61–141	46	34–62

[Ca²⁺] mM	Pulse mV	Rise τ_Rise ms	95% CI	Fall τ_Fall ms	95% CI
20	−100	33	28–38	63	60–67
2	−100	71	62–80	62	58–65
0.6	−100	86	72–103	50	44–58
2	−100	81	69–95	59	53–66
2	−70	96	80–116	55	48–62
2	−40	91	61–141	46	34–62

Changes in Orai1-GCaMP6f fluorescence were recorded in response to test pulses and varying external [Ca²⁺]. n = 6 cells acquired over 4 d. τ_Fall, fall time constant.

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