Table 3.
Orai-GCaMP6f fluorescence rise/fall kinetics
[Ca2+] mMPulse mVRise τRise ms95% CIFall τFall ms95% CI
20 −100 33 28–38 63 60–67 
2 −100 71 62–80 62 58–65 
0.6 −100 86 72–103 50 44–58 
2 −100 81 69–95 59 53–66 
2 −70 96 80–116 55 48–62 
2 −40 91 61–141 46 34–62 
[Ca2+] mMPulse mVRise τRise ms95% CIFall τFall ms95% CI
20 −100 33 28–38 63 60–67 
2 −100 71 62–80 62 58–65 
0.6 −100 86 72–103 50 44–58 
2 −100 81 69–95 59 53–66 
2 −70 96 80–116 55 48–62 
2 −40 91 61–141 46 34–62 

Changes in Orai1-GCaMP6f fluorescence were recorded in response to test pulses and varying external [Ca2+]. n = 6 cells acquired over 4 d. τFall, fall time constant.

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