Table 2. Comparison of biophysical... | Rockefeller University Press

Table 2.

Comparison of biophysical properties of GFP-Orai1 and Orai-GCaMP6f STIM1-operated currents

Construct	t_dev, s	I_Ca2 density, pA/pF	I_Ca20 density, pA/pF	I_Ca20/I_Ca2	Vrev_DVF, mV	I_min/I_max
GFP-Orai1 (6)	193 ± 54^a	8.1 ± 1.1	18.8 ± 2.3	2.37 ± 0.19	66 ± 3	0.39 ± 0.02
Orai1-GCaMP6f (6)	294 ± 88	13.1 ± 2.8	24.4 ± 5.0	1.94 ± 0.14	74 ± 4	0.36 ± 0.02
P value	0.36	0.14	0.34	0.09	0.14	0.30

Construct	t_dev, s	I_Ca2 density, pA/pF	I_Ca20 density, pA/pF	I_Ca20/I_Ca2	Vrev_DVF, mV	I_min/I_max
GFP-Orai1 (6)	193 ± 54^a	8.1 ± 1.1	18.8 ± 2.3	2.37 ± 0.19	66 ± 3	0.39 ± 0.02
Orai1-GCaMP6f (6)	294 ± 88	13.1 ± 2.8	24.4 ± 5.0	1.94 ± 0.14	74 ± 4	0.36 ± 0.02
P value	0.36	0.14	0.34	0.09	0.14	0.30

Biophysical meaning of parameters is presented in Fig. 1. Data presented as mean ± SEM; number of cells used for calculations is indicated in parentheses.

To calculate this value, n = 5.

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