Table 1. External solutions External... | Rockefeller University Press

Table 1.

External solutions

External solutions used in combined TIRF/patch clamp experiments
Name	NaCl	CholineCl	CaCl₂	Sucrose
Ca2	151.5	—	2	8
Ca0	151.5	4	—	8
Ca0.2	151.5	3.6	0.2	8
Ca0.6	151.5	2.8	0.6	8
Ca20	115.5	—	20	28
External solutions used in patch clamp only experiments
Name	NaCl		CaCl₂
Ca2	152		2
Ca20	129		20
Na DVF	160		—

External solutions used in combined TIRF/patch clamp experiments
Name	NaCl	CholineCl	CaCl₂	Sucrose
Ca2	151.5	—	2	8
Ca0	151.5	4	—	8
Ca0.2	151.5	3.6	0.2	8
Ca0.6	151.5	2.8	0.6	8
Ca20	115.5	—	20	28
External solutions used in patch clamp only experiments
Name	NaCl		CaCl₂
Ca2	152		2
Ca20	129		20
Na DVF	160		—

Ca0 solution contained 2 mM EGTA. All solutions in top half contained (in mM) 4.5 KCl, 1 MgCl₂, 10 glucose, and 15 HEPES; pH was adjusted to 7.4 with NaOH; osmolality 325 mOsm. All solutions in bottom half contained (in mM) 10 glucose and 10 HEPES; pH was adjusted to 7.4 with NaOH. Ca2 and Ca20 solutions contained 4.5 mM KCl and 1 mM MgCl₂. Na DVF solution contained 2 mM N-(2-Hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid (HEDTA). Osmolality of all external solutions was 325 mOs.

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