Table 2.
Effects of BK channel blockers on NMJ function in low-Ca2+ solution (0.5 mM Ca2+, 2 mM Mg2+)
Toxin (company)Number of mice (number of NMJs)Results (before vs. after toxin)Nested ANOVA (P)
Iberiotoxin (Alomone), 400 nM 3 (47 before, 51 after) Quantal content, 1.30 ± 0.09 vs.1.35 ± 0.09; mFreq, 6.46 ± 1.68 vs. 8.39 ± 1.63 Quantal content, 0.705; mFreq, 0.412 
Charybdotoxin (Alomone), 400 nM 3 (46 before, 50 after) Quantal content, 1.50 ± 0.13 vs. 1.38 ± 0.12; mFreq, 3.69 ± 1.01 vs. 6.28 ± 0.99 Quantal content, 0.480; mFreq, 0.070 
Paxilline (Sigma-Aldrich), 10 µM 3 (42 before, 43 after) Quantal content, 0.92 ± 0.09 vs. 0.90 ± 0.08; mFreq, 6.41 ± 1.67 vs. 9.45 ± 1.61 Quantal content, 0.918; mFreq, 0.194 
Toxin (company)Number of mice (number of NMJs)Results (before vs. after toxin)Nested ANOVA (P)
Iberiotoxin (Alomone), 400 nM 3 (47 before, 51 after) Quantal content, 1.30 ± 0.09 vs.1.35 ± 0.09; mFreq, 6.46 ± 1.68 vs. 8.39 ± 1.63 Quantal content, 0.705; mFreq, 0.412 
Charybdotoxin (Alomone), 400 nM 3 (46 before, 50 after) Quantal content, 1.50 ± 0.13 vs. 1.38 ± 0.12; mFreq, 3.69 ± 1.01 vs. 6.28 ± 0.99 Quantal content, 0.480; mFreq, 0.070 
Paxilline (Sigma-Aldrich), 10 µM 3 (42 before, 43 after) Quantal content, 0.92 ± 0.09 vs. 0.90 ± 0.08; mFreq, 6.41 ± 1.67 vs. 9.45 ± 1.61 Quantal content, 0.918; mFreq, 0.194 

Results are shown as mean ± SEM. mFreq, frequency of mEPCs in the absence of nerve stimulation.

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