Table 3. Summary of single channel data... | Rockefeller University Press

Table 3.

Summary of single channel data for GluN2A mutations

	Events (n)	Burst P_open	Burst mean open time (ms)	Burst mean shut time (ms)	Shut time T_crit (ms)	n
GluN2A WT	315,130	0.34 ± 0.008	1.5 ± 0.1	2.9 ± 0.2	31 ± 2	6
GluN2A-L550A	6,519	0.033 ± 0.006^a	0.66 ± 0.05^a	22 ± 3	155 ± 13	7
GluN2A-E551A	307,463	0.38 ± 0.04	1.3 ± 0.1	2.3 ±0.3	24 ± 2	3
GluN2A-P552A	17,623	0.34 ± 0.02	1.3 ± 0.06	2.5 ± 0.2	25 ± 3	7
GluN2A-F553A	3,484	0.020 ± 0.005^a	0.21 ± 0.007^a	12 ± 3	59 ± 6	3
GluN2A-A555P	74,905	0.39 ± 0.06	1.7 ± 0.1	2.8 ± 0.5	38 ± 9	3
GluN2A-F553Y	67,723	0.23 ± 0.02^a	1.4 ± 0.15	4.6 ± 0.07	48 ± 5	3

	Events (n)	Burst P_open	Burst mean open time (ms)	Burst mean shut time (ms)	Shut time T_crit (ms)	n
GluN2A WT	315,130	0.34 ± 0.008	1.5 ± 0.1	2.9 ± 0.2	31 ± 2	6
GluN2A-L550A	6,519	0.033 ± 0.006a	0.66 ± 0.05a	22 ± 3	155 ± 13	7
GluN2A-E551A	307,463	0.38 ± 0.04	1.3 ± 0.1	2.3 ±0.3	24 ± 2	3
GluN2A-P552A	17,623	0.34 ± 0.02	1.3 ± 0.06	2.5 ± 0.2	25 ± 3	7
GluN2A-F553A	3,484	0.020 ± 0.005a	0.21 ± 0.007a	12 ± 3	59 ± 6	3
GluN2A-A555P	74,905	0.39 ± 0.06	1.7 ± 0.1	2.8 ± 0.5	38 ± 9	3
GluN2A-F553Y	67,723	0.23 ± 0.02a	1.4 ± 0.15	4.6 ± 0.07	48 ± 5	3

All data are from GluN1 coexpressed with GluN2A. Data expressed as mean ± SEM to two significant figures. P_open, open probability.

P < 0.05 compared with WT GluN2A, one-way ANOVA, Tukey’s post hoc test, corrected for familywise error for P_open and burst mean open time.

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