Table 2.
Effect of 4-AP and ⍺-DTX on charbachol-induced CA2 PN spontaneous activity
MeasurementControl (n = 25)Nonselective block of voltage-gated potassium channels (100 µM 4-AP; n = 8)D-type potassium channels blocked (100 nM ⍺-DTX; n = 9)Statistics
ACSF-VM (mV) −75 ± 1.2 −76 ± 1.5 −71 ± 1.1 P = 0.152a 
CCh-VM (mV) −64 ± 1.0 −66 ± 1.5 −65 ± 0.7 P = 0.613 
ΔACSF-CCh VM (mV) 11 ± 1.0 10 ± 1.5 7 ± 0.9 P = 0.058 
Depolarization rate (mV/min) 6.5 ± 0.8 5.4 ± 0.8 3.4 ± 0.6 P = 0.091 
Burst onset (min) 6.4 ± 0.7 4.4 ± 1.0 6.1 ± 0.9 P = 0.078a 
Interburst interval (s) 100 ± 14.7 119 ± 26.2 136 ± 33.0 P = 0.512a 
Burst-VM (mV) −42 ± 1.0 −37 ± 3.2 −44 ± 0.9 P = 0.067 
Interburst-VM (mV) −62 ± 0.8 −66 ± 1.5 −64 ± 1.2 P = 0.079a 
Δburst-IBI VM (mV) 22 ± 1.3 29 ± 3.2b 20 ± 1.8 P = 0.034a 
Burst rise rate (mV/s) 3.5 ± 0.5 97.5 ± 38.0c 65.6 ± 33.4c P < 0.001 
Burst decay rate (mV/s) −4.6 ± 0.8 −21.6 ± 5.0c −13.3 ± 6.5 P = 0.008 
Burst duration (s) 3.8 ± 0.5 1.9 ± 0.7 6.2 ± 1.9 P = 0.070 
Number of APs/burst 22 ± 2.5 25 ± 7.7 54 ± 19.9 P = 0.266 
Firing frequency in burst (Hz) 13 ± 1.9 30 ± 7.1c 16 ± 3.9 P = 0.027 
MeasurementControl (n = 25)Nonselective block of voltage-gated potassium channels (100 µM 4-AP; n = 8)D-type potassium channels blocked (100 nM ⍺-DTX; n = 9)Statistics
ACSF-VM (mV) −75 ± 1.2 −76 ± 1.5 −71 ± 1.1 P = 0.152a 
CCh-VM (mV) −64 ± 1.0 −66 ± 1.5 −65 ± 0.7 P = 0.613 
ΔACSF-CCh VM (mV) 11 ± 1.0 10 ± 1.5 7 ± 0.9 P = 0.058 
Depolarization rate (mV/min) 6.5 ± 0.8 5.4 ± 0.8 3.4 ± 0.6 P = 0.091 
Burst onset (min) 6.4 ± 0.7 4.4 ± 1.0 6.1 ± 0.9 P = 0.078a 
Interburst interval (s) 100 ± 14.7 119 ± 26.2 136 ± 33.0 P = 0.512a 
Burst-VM (mV) −42 ± 1.0 −37 ± 3.2 −44 ± 0.9 P = 0.067 
Interburst-VM (mV) −62 ± 0.8 −66 ± 1.5 −64 ± 1.2 P = 0.079a 
Δburst-IBI VM (mV) 22 ± 1.3 29 ± 3.2b 20 ± 1.8 P = 0.034a 
Burst rise rate (mV/s) 3.5 ± 0.5 97.5 ± 38.0c 65.6 ± 33.4c P < 0.001 
Burst decay rate (mV/s) −4.6 ± 0.8 −21.6 ± 5.0c −13.3 ± 6.5 P = 0.008 
Burst duration (s) 3.8 ± 0.5 1.9 ± 0.7 6.2 ± 1.9 P = 0.070 
Number of APs/burst 22 ± 2.5 25 ± 7.7 54 ± 19.9 P = 0.266 
Firing frequency in burst (Hz) 13 ± 1.9 30 ± 7.1c 16 ± 3.9 P = 0.027 
a

One-way ANOVA.

b

P = 0.046 vs. CT; Tukey post hoc test.

c

P < 0.05 vs. CT; Dunn–Holland–Wolfe post hoc test.

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