Table 1.
Effect of synaptic transmission on charbachol-induced CA2 PN spontaneous activity
MeasurementControl (n = 25)aExcitatory transmission blocked (n = 7)bInhibitory transmission blocked (n = 6)cExcitatory and inhibitory transmission blocked (n = 6)dStatistics
ACSF-VM before CCh application (mV) −75 ± 1.2 −72 ± 3.1 −80 ± 1.9 −70 ± 4.1 P = 0.054e 
CCh-VM (mV) −64 ± 1.0 −62 ± 2.1 −64 ± 1.5 −57 ± 2.9f P = 0.036e 
ΔACSF-CCh VM (mV) 11 ± 1.0 9.5 ± 1.5 16 ± 1.9 13 ± 1.4 P = 0.065e 
Depolarization rate (mV/min) 6.5 ± 0.8 9.2 ± 3.4 9.4 ± 2.3 8.6 ± 2.0 P = 0.52g 
Burst onset (min) 6.4 ± 0.7 4.6 ± 1.8 4.7 ± 1.2 3.5 ± 0.6 P = 0.21e 
Interburst interval (s) 100 ± 14.7 36 ± 8.3h 113 ± 41.9 36 ± 5.6h P = 0.006g 
Burst-VM (mV) −42 ± 1.0 −36 ± 1.6 −42 ± 2.2 −40 ± 3.2 P = 0.41e 
Interburst-VM (mV) −62 ± 0.8 −56 ± 1.4i −63 ± 1.9 −56 ± 1.1j P < 0.001e 
Δburst-IBI VM (mV) 21 ± 1.2 18 ± 2.6 22 ± 3.7 14 ± 2.3 P = 0.11e 
Burst rise rate (mV/s) 3.5 ± 0.5 5.9 ± 1.1 59 ± 26h 3.6 ± 0.6 P < 0.001g 
Burst decay rate (mV/s) −4.6 ± 0.8 −4.3 ± 0.7 −13.7 ± 4.0 −4.8 ± 0.6 P = 0.087g 
Burst duration (s) 3.8 ± 0.5 2.6 ± 0.7 1.1 ± 0.3h 2.4 ± 0.6 P = 0.037g 
Number of APs/burst 22 ± 2.5 11 ± 1.8h 13 ± 2.3 11 ± 2.5h P = 0.008g 
Firing frequency in burst (Hz) 13 ± 1.9 9.5 ± 1.4 36 ± 7.9h 9.7 ± 4.5 P = 0.012g 
MeasurementControl (n = 25)aExcitatory transmission blocked (n = 7)bInhibitory transmission blocked (n = 6)cExcitatory and inhibitory transmission blocked (n = 6)dStatistics
ACSF-VM before CCh application (mV) −75 ± 1.2 −72 ± 3.1 −80 ± 1.9 −70 ± 4.1 P = 0.054e 
CCh-VM (mV) −64 ± 1.0 −62 ± 2.1 −64 ± 1.5 −57 ± 2.9f P = 0.036e 
ΔACSF-CCh VM (mV) 11 ± 1.0 9.5 ± 1.5 16 ± 1.9 13 ± 1.4 P = 0.065e 
Depolarization rate (mV/min) 6.5 ± 0.8 9.2 ± 3.4 9.4 ± 2.3 8.6 ± 2.0 P = 0.52g 
Burst onset (min) 6.4 ± 0.7 4.6 ± 1.8 4.7 ± 1.2 3.5 ± 0.6 P = 0.21e 
Interburst interval (s) 100 ± 14.7 36 ± 8.3h 113 ± 41.9 36 ± 5.6h P = 0.006g 
Burst-VM (mV) −42 ± 1.0 −36 ± 1.6 −42 ± 2.2 −40 ± 3.2 P = 0.41e 
Interburst-VM (mV) −62 ± 0.8 −56 ± 1.4i −63 ± 1.9 −56 ± 1.1j P < 0.001e 
Δburst-IBI VM (mV) 21 ± 1.2 18 ± 2.6 22 ± 3.7 14 ± 2.3 P = 0.11e 
Burst rise rate (mV/s) 3.5 ± 0.5 5.9 ± 1.1 59 ± 26h 3.6 ± 0.6 P < 0.001g 
Burst decay rate (mV/s) −4.6 ± 0.8 −4.3 ± 0.7 −13.7 ± 4.0 −4.8 ± 0.6 P = 0.087g 
Burst duration (s) 3.8 ± 0.5 2.6 ± 0.7 1.1 ± 0.3h 2.4 ± 0.6 P = 0.037g 
Number of APs/burst 22 ± 2.5 11 ± 1.8h 13 ± 2.3 11 ± 2.5h P = 0.008g 
Firing frequency in burst (Hz) 13 ± 1.9 9.5 ± 1.4 36 ± 7.9h 9.7 ± 4.5 P = 0.012g 
a

10 µM CCh.

b

10 µM CCh, 10 µM NBQX, and 50 µM APV.

c

10 µM CCh, 1 µM SR95531, and 2 µM CGP5584A.

d

10 µM CCh, 10 µM NBQX, 50 µM APV, 1 µM SR95531, and 2 µM CGP5584A.

e

One-way ANOVA.

f

P = 0.025 vs. CT; Tukey post hoc test.

g

Kruskal–Wallis test.

h

P < 0.05 vs. CT; Dunn–Holland–Wolfe post hoc test.

i

P = 0.001 vs. CT; P = 0.013 vs. SR; Tukey post hoc test.

j

P = 0.006 vs. CT; P = 0.033 vs. SR; Tukey post hoc test.

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