Table 2.
Transient charge movement parameters
ConditionRate−100 (s−1)Rate+40 (s−1)V0.5 (mV)Qtot (nC)kT/zqe (mV)Qs/Q (%)n
α1β1 194 ± 21 65 ± 9 −34 ± 5 17 ± 8 34 ± 2 72 ± 6 28 
FXYD1 154 ± 14** 68 ± 6 −43 ± 2** 11 ± 3 34 ± 2 66 ± 4* 
FXYD2 102 ± 19** 67 ± 13 −61 ± 9** 20 ± 8 39 ± 3 59 ± 2** 
FXYD4 251 ± 51** 59 ± 5 −4 ± 9** 18 ± 13 33 ± 1 78 ± 3* 
FXYD6 155 ± 19** 80 ± 12** −54 ± 7** 16 ± 3 37 ± 3 58 ± 4** 16 
FXYD7 167 ± 21* 49 ± 6** −13 ± 4** 16 ± 6 32 ± 4 78 ± 5* 
α1β1−35 mV 156 ± 20 54 ± 4 −30 ± 4 26 ± 7 29 ± 2 77 ± 7 13 
FXYD1−35 mV 120 ± 21** 54 ± 9 −40 ± 7** 18 ± 7 31 ± 2 68 ± 6** 26 
ConditionRate−100 (s−1)Rate+40 (s−1)V0.5 (mV)Qtot (nC)kT/zqe (mV)Qs/Q (%)n
α1β1 194 ± 21 65 ± 9 −34 ± 5 17 ± 8 34 ± 2 72 ± 6 28 
FXYD1 154 ± 14** 68 ± 6 −43 ± 2** 11 ± 3 34 ± 2 66 ± 4* 
FXYD2 102 ± 19** 67 ± 13 −61 ± 9** 20 ± 8 39 ± 3 59 ± 2** 
FXYD4 251 ± 51** 59 ± 5 −4 ± 9** 18 ± 13 33 ± 1 78 ± 3* 
FXYD6 155 ± 19** 80 ± 12** −54 ± 7** 16 ± 3 37 ± 3 58 ± 4** 16 
FXYD7 167 ± 21* 49 ± 6** −13 ± 4** 16 ± 6 32 ± 4 78 ± 5* 
α1β1−35 mV 156 ± 20 54 ± 4 −30 ± 4 26 ± 7 29 ± 2 77 ± 7 13 
FXYD1−35 mV 120 ± 21** 54 ± 9 −40 ± 7** 18 ± 7 31 ± 2 68 ± 6** 26 

Some FXYD1 experiments and some α1β1 controls started from a holding potential of −35 mV in the Dagan amplifier and are shown separately. Errors represent SD; n is the number of determinations. Qtot was determined from the Boltzmann fits to Q-V in individual oocytes (Materials and methods). Qs/Q is the charge of the slow component divided by the steady-state value from the transient-current integral in pulses to +40 mV. Significantly different with respect to α1β1 alone by t test: *P < 0.05; **, P < 0.001.

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