Table 1. Biophysical properties of Na V... | Rockefeller University Press

Table 1.

Biophysical properties of Na_V1.2 variants

Variant	Voltage dependence of activation			Voltage dependence of fast inactivation			Recovery from fast inactivation (−10 mV)
Variant	V_1/2 (mV)	k (mV)	n	V_1/2 (mV)	k (mV)	n	τ_f (ms) (amplitude)	τ_s (ms) (amplitude)	n
Na_V1.2A	−23.5 ± 1.3	6.3 ± 0.3	15	−63.5 ± 0.9	−6.2 ± 0.1	15	1.9 ± 0.1 (0.90 ± 0.01)	275.6 ± 16.4 (0.12 ± 0.01)	15
Na_V1.2A-T236S	−25.6 ± 2.0	7.0 ± 0.3	9	−65.3 ± 1.8	−5.6 ± 0.1	9	2.1 ± 0.1 (0.87 ± 0.01)	270.1 ± 16.8 (0.14 ± 0.01)	8
Na_V1.2A-E999K	−4.6 ± 1.4	6.5 ± 0.3	10	−63.1 ± 1.2	−6.2 ± 0.1	10	1.9 ± 0.2 (0.90 ± 0.01)	269.3 ± 21.2 (0.14 ± 0.01)	10
Na_V1.2A-S1336Y	−23.2 ± 1.4	7.9 ± 0.4	11	−57.7 ± 1.3^a	−6.6 ± 0.3	11	1.3 ± 0.2 (0.84 ± 0.03)	210.3 ± 20.4 (0.21 ± 0.02)	11
Na_V1.2A-T1623N	−18.4 ± 1.4^a	6.4 ± 0.6	8	−56.7 ± 0.8^a	−6.0 ± 0.1	8	2.1 ± 0.1 (0.92 ± 0.01)	240.4 ± 17.4 (0.09 ± 0.01)	8
Na_V1.2A-R1882Q	−19.5 ± 0.9	6.7 ± 0.3	15	−51.2 ± 1.5^a	−7.6 ± 0.3^a	15	1.6 ± 0.1 (0.84 ± 0.02)	248.2 ± 22.0 (0.16 ± 0.02)	15
Na_V1.2N	−19.5 ± 0.8^b	7.0 ± 0.3	15	−62.4 ± 1.2	−6.4 ± 0.1	15	1.7± 0.1 (0.90 ± 0.01)	267.3 ± 28.8 (0.13 ± 0.01)	15
Na_V1.2N-T236S	−29.9 ± 1.1^a	5.7 ± 0.4^a	15	−62.3 ± 0.8	6.1 ± 0.4	8	1.9 ± 0.1 (0.87 ± 0.01)	245.3 ± 7.7 (0.14 ± 0.01)	7
Na_V1.2N-E999K	−26.2 ± 1.7^a	6.3 ± 0.4	14	−63.1 ± 1.3	−5.8 ± 0.1	13	1.8 ± 0.1 (0.89 ± 0.01)	263.2 ± 22.9 (0.12 ± 0.01)	13
Na_V1.2N-S1336Y	−25.3 ± 1.9^a	7.3 ± 0.7	10	−56.9 ± 1.0^a	−7.9 ± 0.8^a	10	1.0 ± 0.1 (0.89 ± 0.02)	261.0 ± 38.8 (0.16 ± 0.03)	10
Na_V1.2N-T1623N	−18.4 ± 1.4	6.2 ± 0.3	9	−56.3 ± 0.7^a	−6.3 ± 0.4	9	2.0 ± 0.1 (0.87 ± 0.02)	213.6 ± 23.9 (0.14 ± 0.02)	9
Na_V1.2N-R1882Q	−20.6 ± 1.7	6.2 ± 0.4	14	−53.4 ± 1.3^a	−7.0 ± 0.2^a	14	1.6 ± 0.1 (0.88 ± 0.02)	258.2 ± 27.8 (0.12 ± 0.02)	14

Variant	Voltage dependence of activation			Voltage dependence of fast inactivation			Recovery from fast inactivation (−10 mV)
Variant	V_1/2 (mV)	k (mV)	n	V_1/2 (mV)	k (mV)	n	τ_f (ms) (amplitude)	τ_s (ms) (amplitude)	n
Na_V1.2A	−23.5 ± 1.3	6.3 ± 0.3	15	−63.5 ± 0.9	−6.2 ± 0.1	15	1.9 ± 0.1 (0.90 ± 0.01)	275.6 ± 16.4 (0.12 ± 0.01)	15
Na_V1.2A-T236S	−25.6 ± 2.0	7.0 ± 0.3	9	−65.3 ± 1.8	−5.6 ± 0.1	9	2.1 ± 0.1 (0.87 ± 0.01)	270.1 ± 16.8 (0.14 ± 0.01)	8
Na_V1.2A-E999K	−4.6 ± 1.4	6.5 ± 0.3	10	−63.1 ± 1.2	−6.2 ± 0.1	10	1.9 ± 0.2 (0.90 ± 0.01)	269.3 ± 21.2 (0.14 ± 0.01)	10
Na_V1.2A-S1336Y	−23.2 ± 1.4	7.9 ± 0.4	11	−57.7 ± 1.3^a	−6.6 ± 0.3	11	1.3 ± 0.2 (0.84 ± 0.03)	210.3 ± 20.4 (0.21 ± 0.02)	11
Na_V1.2A-T1623N	−18.4 ± 1.4^a	6.4 ± 0.6	8	−56.7 ± 0.8^a	−6.0 ± 0.1	8	2.1 ± 0.1 (0.92 ± 0.01)	240.4 ± 17.4 (0.09 ± 0.01)	8
Na_V1.2A-R1882Q	−19.5 ± 0.9	6.7 ± 0.3	15	−51.2 ± 1.5^a	−7.6 ± 0.3^a	15	1.6 ± 0.1 (0.84 ± 0.02)	248.2 ± 22.0 (0.16 ± 0.02)	15
Na_V1.2N	−19.5 ± 0.8^b	7.0 ± 0.3	15	−62.4 ± 1.2	−6.4 ± 0.1	15	1.7± 0.1 (0.90 ± 0.01)	267.3 ± 28.8 (0.13 ± 0.01)	15
Na_V1.2N-T236S	−29.9 ± 1.1^a	5.7 ± 0.4^a	15	−62.3 ± 0.8	6.1 ± 0.4	8	1.9 ± 0.1 (0.87 ± 0.01)	245.3 ± 7.7 (0.14 ± 0.01)	7
Na_V1.2N-E999K	−26.2 ± 1.7^a	6.3 ± 0.4	14	−63.1 ± 1.3	−5.8 ± 0.1	13	1.8 ± 0.1 (0.89 ± 0.01)	263.2 ± 22.9 (0.12 ± 0.01)	13
Na_V1.2N-S1336Y	−25.3 ± 1.9^a	7.3 ± 0.7	10	−56.9 ± 1.0^a	−7.9 ± 0.8^a	10	1.0 ± 0.1 (0.89 ± 0.02)	261.0 ± 38.8 (0.16 ± 0.03)	10
Na_V1.2N-T1623N	−18.4 ± 1.4	6.2 ± 0.3	9	−56.3 ± 0.7^a	−6.3 ± 0.4	9	2.0 ± 0.1 (0.87 ± 0.02)	213.6 ± 23.9 (0.14 ± 0.02)	9
Na_V1.2N-R1882Q	−20.6 ± 1.7	6.2 ± 0.4	14	−53.4 ± 1.3^a	−7.0 ± 0.2^a	14	1.6 ± 0.1 (0.88 ± 0.02)	258.2 ± 27.8 (0.12 ± 0.02)	14

P < 0.05 between WT and variant in same splice isoform.

P < 0.05 between neonatal and adult isoforms.