Table 1. Summary of measured parameters... | Rockefeller University Press

Table 1.

Summary of measured parameters for hV3.

Parameter measured		Protein characteristic or process that is monitored
ME	ME₂₁₅	Secondary structure content
T_m	ME₂₁₅	Stability of folded state
T_m	A₃₄₀	Stability of folded monomer; aggregation tendency
T_m-start	ME₂₁₅	Stability, temperature at which protein unfolding is nucleated
T_m-start	A₃₄₀	Stability, temperature at which protein aggregation is nucleated
T_m-end	ME₂₁₅	Stability, end-point temperature of protein unfolding
T_m-end	A₃₄₀	Stability, end-point temperature of protein aggregation
ΔT_m	ME₂₁₅	Indirect measure of rate of unfolding and aggregation
ΔT_m	A₃₄₀	Indirect measure of rate of aggregation
ΔH_ME-app	ME₂₁₅	Apparent unfolding enthalpy; indirect measure of unfolding cooperativity
E_act	ME₂₁₅	Stability, activation energy of unfolding and aggregation
	A₃₄₀	Stability, activation energy of aggregation
	HT₂₁₅	Stability, activation energy of aggregation
AI	A₃₂₀	Aggregation propensity
AI	A₃₄₀	Aggregation propensity

Parameter measured		Protein characteristic or process that is monitored
ME	ME₂₁₅	Secondary structure content
T_m	ME₂₁₅	Stability of folded state
T_m	A₃₄₀	Stability of folded monomer; aggregation tendency
T_m-start	ME₂₁₅	Stability, temperature at which protein unfolding is nucleated
T_m-start	A₃₄₀	Stability, temperature at which protein aggregation is nucleated
T_m-end	ME₂₁₅	Stability, end-point temperature of protein unfolding
T_m-end	A₃₄₀	Stability, end-point temperature of protein aggregation
ΔT_m	ME₂₁₅	Indirect measure of rate of unfolding and aggregation
ΔT_m	A₃₄₀	Indirect measure of rate of aggregation
ΔH_ME-app	ME₂₁₅	Apparent unfolding enthalpy; indirect measure of unfolding cooperativity
E_act	ME₂₁₅	Stability, activation energy of unfolding and aggregation
	A₃₄₀	Stability, activation energy of aggregation
	HT₂₁₅	Stability, activation energy of aggregation
AI	A₃₂₀	Aggregation propensity
AI	A₃₄₀	Aggregation propensity

ME, molar ellipticity measured using CD spectropolarimetry; A₃₄₀ or A₃₂₀, absorbance measured using UV absorbance spectroscopy at 340 nm or 320 nm, respectively.