Table 2.
Concentration–response data for CTP-gated currents at different mutants
rP2X2 constructEC50 (µM)nHn
WT 370 ± 30 1.7 ± 0.2 7 
Val70Vah 2,000 ± 90**** 2.6 ± 0.1**** 6 
Thr184Ser 156 ± 30 1.5 ± 0.1 6 
Thr184Val 11,700 ± 300**** 1.6 ± 0.1 8 
Ile185Val 144 ± 30 1.3 ± 0.1 6 
Ile185Vah 1,100 ± 80* 1.7 ± 0.1* 7 
rP2X2 constructEC50 (µM)nHn
WT 370 ± 30 1.7 ± 0.2 7 
Val70Vah 2,000 ± 90**** 2.6 ± 0.1**** 6 
Thr184Ser 156 ± 30 1.5 ± 0.1 6 
Thr184Val 11,700 ± 300**** 1.6 ± 0.1 8 
Ile185Val 144 ± 30 1.3 ± 0.1 6 
Ile185Vah 1,100 ± 80* 1.7 ± 0.1* 7 

CTP-elicited concentration–response data (EC50) and Hill coefficient (nH; shown as mean ± SEM), as well as number of experiments (n) for WT, the conventional Thr184Ser, Thr184Val, Ile185Val, and the Val70Vah/Ile185Vah amide-to-ester mutants. Significant differences were determined by one-way ANOVA with Dunnett’s test. *, P ≤ 0.01; ****, P < 0.0001.

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