| rP2X2 construct . | EC50 (µM) . | nH . | n . |
|---|---|---|---|
| WT | 32 ± 4 | 1.4 ± 0.1 | 15 |
| Lys69Arg | 6,000 ± 500**** | 1.2 ± 0.1 | 13 |
| Lys69Gln | 16,600 ± 550**** | 2.4 ± 0.1**** | 10 |
| Val70Vah | 120 ± 2 | 1.6 ± 0.1 | 12 |
| Val96Vah | 27 ± 2 | 1.7 ± 0.1 | 6 |
| Lys71Arg | 370 ± 40 | 1.1 ± 0.1 | 12 |
| Lys71Gln | 6,300 ± 400**** | 1.4 ± 0.1 | 15 |
| Lys188Arg | 57 ± 8 | 1.1 ± 0.1** | 13 |
| Lys188Gln | 8,700 ± 500**** | 1.7 ± 0.1 | 16 |
| Lys308Arg | 6,000 ± 400**** | 0.8 ±0.1**** | 24 |
| Lys308Gln | 5,800 ± 700**** | 1.1 ±0.1 | 13 |
| Arg290Lys | 460 ± 60 | 1 ± 0.1** | 7 |
| Arg290Gln | 4,900 ± 500**** | 1.1 ± 0.1** | 15 |
| rP2X2 construct . | EC50 (µM) . | nH . | n . |
|---|---|---|---|
| WT | 32 ± 4 | 1.4 ± 0.1 | 15 |
| Lys69Arg | 6,000 ± 500**** | 1.2 ± 0.1 | 13 |
| Lys69Gln | 16,600 ± 550**** | 2.4 ± 0.1**** | 10 |
| Val70Vah | 120 ± 2 | 1.6 ± 0.1 | 12 |
| Val96Vah | 27 ± 2 | 1.7 ± 0.1 | 6 |
| Lys71Arg | 370 ± 40 | 1.1 ± 0.1 | 12 |
| Lys71Gln | 6,300 ± 400**** | 1.4 ± 0.1 | 15 |
| Lys188Arg | 57 ± 8 | 1.1 ± 0.1** | 13 |
| Lys188Gln | 8,700 ± 500**** | 1.7 ± 0.1 | 16 |
| Lys308Arg | 6,000 ± 400**** | 0.8 ±0.1**** | 24 |
| Lys308Gln | 5,800 ± 700**** | 1.1 ±0.1 | 13 |
| Arg290Lys | 460 ± 60 | 1 ± 0.1** | 7 |
| Arg290Gln | 4,900 ± 500**** | 1.1 ± 0.1** | 15 |
ATP-elicited concentration–response data (EC50) and Hill coefficient (nH; shown as mean ± SEM), as well as number of experiments (n) for WT and mutants of conserved basic amino acids (including Val70Vah and Val96Vah amide-to-ester mutant, the latter serving as a control for Vah incorporation). Significant differences were determined by one-way ANOVA with Dunnett’s test. **, P < 0.01; ****, P < 0.0001.