Table 3. Effect of mutations on ΔG 2 Cho... | Rockefeller University Press

Table 3.

Effect of mutations on ΔG₂^Cho in human AChRs

Mutation	f₂(s⁻¹)	b₂(s⁻¹)	E₂^Cho	E₂^mut/Cho/E₂^WT	ΔΔG₂^Cho (kcal/mol)
—	76	2,252	0.034	1	0
αE45R	4,002	2,095	1.91	56	−2.4
αA96V	3,380	885	3.82	112	−2.8
αD97A	6,624	1,533	4.3	126	−2.8
αY127F	3,471	1,001	3.47	102	−2.7
αS266E	30	3,023	0.01	0.30	0.7
αS269I	1,832	706	2.6	77	−2.6
αP272A	1,458	236	6.2	182	−3.1
αC418W	731	184	3.98	116	−2.8
βL262T	844	675	1.25	36	−2.1
βV266A	424	28	15.1	445	−3.6
βT456I	101	826	0.14	3.6	−0.8
βT456F	314	342	0.92	27	−1.9
δI43Q	200	1,893	0.105	3.1	−0.7
δI43H	20.5	5,067	0.004	0.12	1.3
δL265T	190	148	1.48	37	−2.1
δL265S	172	9.2	18.7	550	−3.7
εL261S	1,956	134	14.6	429	−3.6
εL269F	831	197	4.2	124	−2.8

Mutation	f₂(s⁻¹)	b₂(s⁻¹)	E₂^Cho	E₂^mut/Cho/E₂^WT	ΔΔG₂^Cho (kcal/mol)
—	76	2,252	0.034	1	0
αE45R	4,002	2,095	1.91	56	−2.4
αA96V	3,380	885	3.82	112	−2.8
αD97A	6,624	1,533	4.3	126	−2.8
αY127F	3,471	1,001	3.47	102	−2.7
αS266E	30	3,023	0.01	0.30	0.7
αS269I	1,832	706	2.6	77	−2.6
αP272A	1,458	236	6.2	182	−3.1
αC418W	731	184	3.98	116	−2.8
βL262T	844	675	1.25	36	−2.1
βV266A	424	28	15.1	445	−3.6
βT456I	101	826	0.14	3.6	−0.8
βT456F	314	342	0.92	27	−1.9
δI43Q	200	1,893	0.105	3.1	−0.7
δI43H	20.5	5,067	0.004	0.12	1.3
δL265T	190	148	1.48	37	−2.1
δL265S	172	9.2	18.7	550	−3.7
εL261S	1,956	134	14.6	429	−3.6
εL269F	831	197	4.2	124	−2.8

E₂ = f₂/b₂. ΔΔG₂^Cho, gating free energy change with two bound Cho molecules; f₂ and b₂, diliganded forward and backward gating rate constants.