Table 1. Cardiac muscle simulation... | Rockefeller University Press

Table 1.

Cardiac muscle simulation parameters for various experimental protocols modulating titin contribution to passive muscle fiber elasticity, 3-D sarcomere geometry, and kinetics of myosin binding to actin

SL	Protocol	Active tension		Active force	Passive tension		Passive force	Passive stiffness	d_1,0	k_bind	$Δ G_{b i n d}$	# XB
(µm)		(mN/mm²)		(pN/MyoF)	(mN/mm²)		(pN/MyoF)	(pN/nm/MyoF)	(nm)	(s⁻¹)	(k_BT)
2.0	LPT (Mech)	30.7	±2.3	98.65	0.5	±0.1	1.61	0.03213	42.856	12.0	0.482	55.13
2.3	LPT (Mech)	31.6	±1.7	101.31	1.7	±0.3	5.46	0.02731	40.902	17.8	0.876	59.67
2.0	LPT	30.7	±2.3	98.65	0.6	±0.1	1.93	0.03856	42.856	12.0	0.482	55.11
2.3	HPT	34.3	±1.8	110.21	8.7	±0.3	27.95	0.13977	40.902	21.0	1.042	60.92
2.3	LPT (Trypsin)	33.1	±2.8	106.36	1.7	±0.6	5.46	0.02731	43.668	20.1	0.998	60.56
1.9	LPT	25.7	±2.0	82.58	∼0		0.00	0.00000	43.504	8.2	0.101	51.29
1.9	LPT (Trypsin)	23.3	±1.7	74.87	∼0		0.00	0.00000	47.336	6.5	-0.131	50.08
2.0	LPT (Mech) +Dextran	32.4	±2.5	104.11	0.4	±0.1	1.29	0.02571	35.599	12.8	0.547	59.23
2.3	LPT (Mech) + Dextran	34.9	±2.0	112.14	2.2	±0.4	7.07	0.03535	35.003	24.5	1.196	64.71
2.0	LPT + Dextran	32.4	±2.5	104.11	0.5	±0.1	1.61	0.03213	35.599	12.2	0.499	58.73
2.3	HPT + Dextran	36.6	±2.3	117.60	8.7	±0.9	27.95	0.13977	35.003	27.1	1.297	66.29

SL	Protocol	Active tension		Active force	Passive tension		Passive force	Passive stiffness	d_1,0	k_bind	$Δ G_{b i n d}$	# XB
(µm)		(mN/mm²)		(pN/MyoF)	(mN/mm²)		(pN/MyoF)	(pN/nm/MyoF)	(nm)	(s⁻¹)	(k_BT)
2.0	LPT (Mech)	30.7	±2.3	98.65	0.5	±0.1	1.61	0.03213	42.856	12.0	0.482	55.13
2.3	LPT (Mech)	31.6	±1.7	101.31	1.7	±0.3	5.46	0.02731	40.902	17.8	0.876	59.67
2.0	LPT	30.7	±2.3	98.65	0.6	±0.1	1.93	0.03856	42.856	12.0	0.482	55.11
2.3	HPT	34.3	±1.8	110.21	8.7	±0.3	27.95	0.13977	40.902	21.0	1.042	60.92
2.3	LPT (Trypsin)	33.1	±2.8	106.36	1.7	±0.6	5.46	0.02731	43.668	20.1	0.998	60.56
1.9	LPT	25.7	±2.0	82.58	∼0		0.00	0.00000	43.504	8.2	0.101	51.29
1.9	LPT (Trypsin)	23.3	±1.7	74.87	∼0		0.00	0.00000	47.336	6.5	-0.131	50.08
2.0	LPT (Mech) +Dextran	32.4	±2.5	104.11	0.4	±0.1	1.29	0.02571	35.599	12.8	0.547	59.23
2.3	LPT (Mech) + Dextran	34.9	±2.0	112.14	2.2	±0.4	7.07	0.03535	35.003	24.5	1.196	64.71
2.0	LPT + Dextran	32.4	±2.5	104.11	0.5	±0.1	1.61	0.03213	35.599	12.2	0.499	58.73
2.3	HPT + Dextran	36.6	±2.3	117.60	8.7	±0.9	27.95	0.13977	35.003	27.1	1.297	66.29

Abbreviations of the protocols reported by Cazorla et al. (2001): Dextran, reduced filament spacing by osmotic compression with 2.5% dextran in skinned muscle preparation; Mech, mechanical protocol; Trypsin, skinned muscle preparations in which the titin had been degraded by 0.25 mg/ml trypsin for 25 min at 25°C. In all simulations, k_–bind = 7.41 s⁻¹ is used. Number of bound cross-bridges per half myosin filament denoted as # XB. Total number of crossbridges per half myosin filament is 150.

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