| qPCR primers . | . | . | . | . |
|---|---|---|---|---|
| Gene . | Accession no. . | . | Catalog Number . | Description . |
| Ano1 | NM_178642 | IDT Mm.PT.58.12522115 | Mouse Ano1 TaqMan probe IDT | |
| SMA | NM_007392 | IDT Mm.PT.58.16320644 | Mouse α-actin TaqMan probe IDT | |
| β-Actin | NM_007393 | IDT Mm.PT.58.33257376.gs | Mouse Actb TaqMan probe IDT | |
| End point RT-PCR | ||||
| Gene | Accession no. | Strand | Sequence / Catalog Number | Amplicon (bp) |
| Ano1 | NM_178642 | F | 5′-GGCATTTGTCATTGTCTTCCAG-3′ | 141 |
| R | 5′-TCCTCACGCATAAACAGCTC-3′ | |||
| TMEM16B | NM_153589 | F | 5′-TTTATGATTGCCCTGACGTTCTC-3′ | 141 |
| R | 5′-GAGGTTGATGATGACTGCTGTT-3′ | |||
| Bestrophin 1 | NM_011913 | F | 5′-ACACAACACATTCTGGGTGC-3′ | 123 |
| R | 5′-CGCAAAGTACACACCTCATTCA-3′ | |||
| Bestrophin 2 | NM_001130194.1 | F | 5′-TCCAGGGCTCCACCTTTGATATAG-3′ | 101 |
| R | 5′-AAAGTCCCCGTGAACCTCTCC-3′ | |||
| CLCA1 | NM_017474 | F | 5′-GAAATTCCCCAGCCCTGTAACAG-3′ | 144 |
| R | 5′-TCGGCACCTGCTCCGTTATC-3′ | |||
| CLCA3A1 | NM_009899 | F | 5′-GTGGACCAGCCTTTCTACATGTCTAG-3′ | 114 |
| R | 5′-TGTGACACAGTTGCCTCTCTCA-3′ | |||
| NKCC1 (Slc12a2) | NM_009194 | F | IDT Mm.PT.58.32659494 | 131 |
| R | ||||
| NKCC2 (Slc12a1) | NM_001079690 | F | IDT Mm.PT.58.29984258 | 118 |
| R | ||||
| qPCR primers . | . | . | . | . |
|---|---|---|---|---|
| Gene . | Accession no. . | . | Catalog Number . | Description . |
| Ano1 | NM_178642 | IDT Mm.PT.58.12522115 | Mouse Ano1 TaqMan probe IDT | |
| SMA | NM_007392 | IDT Mm.PT.58.16320644 | Mouse α-actin TaqMan probe IDT | |
| β-Actin | NM_007393 | IDT Mm.PT.58.33257376.gs | Mouse Actb TaqMan probe IDT | |
| End point RT-PCR | ||||
| Gene | Accession no. | Strand | Sequence / Catalog Number | Amplicon (bp) |
| Ano1 | NM_178642 | F | 5′-GGCATTTGTCATTGTCTTCCAG-3′ | 141 |
| R | 5′-TCCTCACGCATAAACAGCTC-3′ | |||
| TMEM16B | NM_153589 | F | 5′-TTTATGATTGCCCTGACGTTCTC-3′ | 141 |
| R | 5′-GAGGTTGATGATGACTGCTGTT-3′ | |||
| Bestrophin 1 | NM_011913 | F | 5′-ACACAACACATTCTGGGTGC-3′ | 123 |
| R | 5′-CGCAAAGTACACACCTCATTCA-3′ | |||
| Bestrophin 2 | NM_001130194.1 | F | 5′-TCCAGGGCTCCACCTTTGATATAG-3′ | 101 |
| R | 5′-AAAGTCCCCGTGAACCTCTCC-3′ | |||
| CLCA1 | NM_017474 | F | 5′-GAAATTCCCCAGCCCTGTAACAG-3′ | 144 |
| R | 5′-TCGGCACCTGCTCCGTTATC-3′ | |||
| CLCA3A1 | NM_009899 | F | 5′-GTGGACCAGCCTTTCTACATGTCTAG-3′ | 114 |
| R | 5′-TGTGACACAGTTGCCTCTCTCA-3′ | |||
| NKCC1 (Slc12a2) | NM_009194 | F | IDT Mm.PT.58.32659494 | 131 |
| R | ||||
| NKCC2 (Slc12a1) | NM_001079690 | F | IDT Mm.PT.58.29984258 | 118 |
| R | ||||
List of all the primer sequences, their corresponding gene accession no. used for their design, and the predicted amplicon size. F, forward; R, reverse.