Table 2. Distance distributions observed... | Rockefeller University Press

Table 2.

Distance distributions observed in cTnC(13c/51c)_AEDANS-DDPM reconstituted fibers under different biochemical conditions

XB state	pCa	WT				n^{^a}	Titin RBM20				n^{^a}
XB state	pCa	1.8 µm SL		2.2 µm SL			1.8 µm SL		2.2 µm SL
		r^{^b} (Å)	FWHM^{^c} (Å)	r^{^b} (Å)	FWHM^{^c} (Å)		r^{^b} (Å)	FWHM^{^c} (Å)	r^{^b} (Å)	FWHM^{^c} (Å)
5 mM ATP	9.0	12.98±0.24	5.83± 0.29	13.83 ± 0.25§	7.19 ± 0.26§	8	12.45 ± 0.32	5.72 ± 0.62	12.95 ± 0.45	5.82 ± 0.51	7
	4.3	21.67±0.17	6.50± 0.37	23.51 ± 0.31^§§	8.91± 0.68^§§		21.01 ± 0.39	4.68 ± 0.73	21.50 ± 0.35†	5.27 ± 0.36^††
5 mM ADP^{^d}	9.0	13.70±0.24	5.92± 0.25	14.87± 0.23§*	6.82± 0.27§	8	13.04 ± 0.34	5.81 ± 0.32	13.68 ± 0.49†	5.60 ± 0.28†	7
	4.3	24.46±0.23	5.72± 0.59	26.34± 0.36^§§**	6.30 ± 0.37**		22.27 ± 0.66^††*	4.57 ± 0.42	23.11 ± 0.55^††**	4.77 ± 0.36†
1 mM Vi^{^e}	9.0	12.03±0.46	5.43± 0.28	12.94 ± 0.33	5.53 ± 0.38**	6	11.82 ± 0.44	6.03 ± 0.33	11.75 ± 0.39	5.92 ± 0.25	6
	4.3	20.09±0.45	5.82± 0.47	21.51 ± 0.57§*	5.95 ± 0.42**		20.28 ± 0.86	5.81 ± 0.35	20.40 ± 0.45†*	6.28 ± 0.38**

XB state	pCa	WT				n^{^a}	Titin RBM20				n^{^a}
XB state	pCa	1.8 µm SL		2.2 µm SL			1.8 µm SL		2.2 µm SL
		r^{^b} (Å)	FWHM^{^c} (Å)	r^{^b} (Å)	FWHM^{^c} (Å)		r^{^b} (Å)	FWHM^{^c} (Å)	r^{^b} (Å)	FWHM^{^c} (Å)
5 mM ATP	9.0	12.98±0.24	5.83± 0.29	13.83 ± 0.25§	7.19 ± 0.26§	8	12.45 ± 0.32	5.72 ± 0.62	12.95 ± 0.45	5.82 ± 0.51	7
	4.3	21.67±0.17	6.50± 0.37	23.51 ± 0.31^§§	8.91± 0.68^§§		21.01 ± 0.39	4.68 ± 0.73	21.50 ± 0.35†	5.27 ± 0.36^††
5 mM ADP^{^d}	9.0	13.70±0.24	5.92± 0.25	14.87± 0.23§*	6.82± 0.27§	8	13.04 ± 0.34	5.81 ± 0.32	13.68 ± 0.49†	5.60 ± 0.28†	7
	4.3	24.46±0.23	5.72± 0.59	26.34± 0.36^§§**	6.30 ± 0.37**		22.27 ± 0.66^††*	4.57 ± 0.42	23.11 ± 0.55^††**	4.77 ± 0.36†
1 mM Vi^{^e}	9.0	12.03±0.46	5.43± 0.28	12.94 ± 0.33	5.53 ± 0.38**	6	11.82 ± 0.44	6.03 ± 0.33	11.75 ± 0.39	5.92 ± 0.25	6
	4.3	20.09±0.45	5.82± 0.47	21.51 ± 0.57§*	5.95 ± 0.42**		20.28 ± 0.86	5.81 ± 0.35	20.40 ± 0.45†*	6.28 ± 0.38**

Absolute parameter values are given as mean ± SEM. Statistical significance level set at P < 0.05 (§, †, and *) and P < 0.01 (^§§, ††, and **) for the following: §, comparing to 1.8 µm sarcomere length at each condition; †, comparing to WT at the same cross-bridge condition, sarcomere length, and Ca²⁺ activation state; and *, comparing to ATP condition at the same sarcomere length and Ca²⁺ activation state. pCa, −log₁₀ of free Ca²⁺ concentration; SL, sarcomere length.

Number of fibers used in each set of measurements.

r is the mean distance associated with the distribution.

FWHM denotes the full width at half maximum.

ATP was absent in ADP solutions.

Vi solutions also contained 5 mM ATP.

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