Table 1. Tension values (mean ± SEM)... | Rockefeller University Press

Table 1.

Tension values (mean ± SEM) from FRET donor–acceptor reconstituted fibers measured at pCa 9 and 4.3 as a function of XB states and sarcomere lengths

XB state	pCa	WT tension (mN mm⁻²)			RBM20-deficient tension (mN mm⁻²)
XB state	pCa	1.8 µm SL	2.2 µm SL	n^{^a}	1.8 µm SL	2.2 µm SL	n^{^a}
5 mM ATP	9.0	2.10 ± 0.00	5.50 ± 0.10^{^b}	8	1.35 ± 0.35^{^c}	2.10 ± 0.00^{^b}^,^{^c}	7
	4.3	20.65 ± 1.55	34.10 ± 0.80^{^b}		17.65 ± 1.65^{^c}	26.55 ± 0.55^{^b}^,^{^c}
5 mM ADP^{^d}	9.0	12.87 ± 2.70	37.30 ± 4.12^{^b}	8	8.30 ± 0.90	28.03 ± 5.02^{^b}^,^{^c}	7
	4.3	17.85 ± 1.95	37.55 ± 0.25^{^b}		12.85 ± 0.35^{^c}	26.45 ± 0.35^{^b}^,^{^c}
1 mM Vi^{^e}	9.0	2.05 ± 1.55	8.90 ± 0.30^{^b}	6	1.40 ± 1.20	5.20 ± 1.30^{^b}^,^{^c}	6
	4.3	2.35 ± 0.15	11.90 ± 0.50^{^b}		1.75 ± 0.55	7.45 ± 0.85^{^b}^,^{^c}

XB state	pCa	WT tension (mN mm⁻²)			RBM20-deficient tension (mN mm⁻²)
XB state	pCa	1.8 µm SL	2.2 µm SL	n^{^a}	1.8 µm SL	2.2 µm SL	n^{^a}
5 mM ATP	9.0	2.10 ± 0.00	5.50 ± 0.10^{^b}	8	1.35 ± 0.35^{^c}	2.10 ± 0.00^{^b}^,^{^c}	7
	4.3	20.65 ± 1.55	34.10 ± 0.80^{^b}		17.65 ± 1.65^{^c}	26.55 ± 0.55^{^b}^,^{^c}
5 mM ADP^{^d}	9.0	12.87 ± 2.70	37.30 ± 4.12^{^b}	8	8.30 ± 0.90	28.03 ± 5.02^{^b}^,^{^c}	7
	4.3	17.85 ± 1.95	37.55 ± 0.25^{^b}		12.85 ± 0.35^{^c}	26.45 ± 0.35^{^b}^,^{^c}
1 mM Vi^{^e}	9.0	2.05 ± 1.55	8.90 ± 0.30^{^b}	6	1.40 ± 1.20	5.20 ± 1.30^{^b}^,^{^c}	6
	4.3	2.35 ± 0.15	11.90 ± 0.50^{^b}		1.75 ± 0.55	7.45 ± 0.85^{^b}^,^{^c}

pCa, −log₁₀ of free Ca²⁺ concentration; SL, sarcomere length.

Number of fibers used in each set of measurements.

Compared with 1.8 µm sarcomere length at each condition (P < 0.05).

Compared with WT at the same cross-bridge condition, sarcomere length, and Ca²⁺ activation state (P < 0.05).

ATP was absent in ADP solutions.

Vi solutions also contained 5 mM ATP.