| Parameter . | Value . | Rationale for parameter . |
|---|---|---|
| Free PI(4,5)P2 (density) | 5,000 µm−2 | Xu et al., 2003 |
| Bound PI(4,5)P2 (density) | 10,000 µm−2 | To make total 15,000 µm−2 |
| PI(4)P (density) | 4,000 µm−2 | Xu et al., 2003 |
| PI (density)(held constant) | 140,000 µm−2 | Xu et al., 2003 |
| PI(3,4,5)P3 (density) | 1,338 µm−2 | FRET response PH-Btk |
| PI(3,4)P2 (density) | 1,200 µm−2 | Steady state of basal PLC and DAGase |
| k_VSP (5Pase on PI(4,5)P2) | 8.0 s−1 | Based on PH-PLCδ1 FRET |
| k_VSP (5Pase on PI(3,4,5)P3) | 2.0 s−1 | Based on PH-Btk and PH-TAPP1 FRET |
| k_VSP (3Pase on PI(3,4)P2) | 0.15 s−1 | Based on PH-TAPP1 and P4M FRET |
| k_VSP (3Pase on PI(3,4,5)P3) | 44.0 s−1 | Based on PH-Btk and PH-PLCδ1 FRET |
| PH-TAPP1-PI(3,4)P2 | 213 µm−2 | Based on FRET |
| PH-TAPP1 (concentration) | 1 µM | Based on fluorescence intensity |
| KD_TAPP-1 | 0.1 µM | Based on FRET and Dowler et al., 2000 |
| V_0.5_Ci-VSP | 57 mV/95 mV | Villalba-Galea et al., 2013, Fig. 2 C/revised here for Fig. 2 D |
| z_Ci-VSP | 1.56/1.4 | Villalba-Galea et al., 2013, Fig. 2 C/revised here for Fig. 2 D |
| Parameter . | Value . | Rationale for parameter . |
|---|---|---|
| Free PI(4,5)P2 (density) | 5,000 µm−2 | Xu et al., 2003 |
| Bound PI(4,5)P2 (density) | 10,000 µm−2 | To make total 15,000 µm−2 |
| PI(4)P (density) | 4,000 µm−2 | Xu et al., 2003 |
| PI (density)(held constant) | 140,000 µm−2 | Xu et al., 2003 |
| PI(3,4,5)P3 (density) | 1,338 µm−2 | FRET response PH-Btk |
| PI(3,4)P2 (density) | 1,200 µm−2 | Steady state of basal PLC and DAGase |
| k_VSP (5Pase on PI(4,5)P2) | 8.0 s−1 | Based on PH-PLCδ1 FRET |
| k_VSP (5Pase on PI(3,4,5)P3) | 2.0 s−1 | Based on PH-Btk and PH-TAPP1 FRET |
| k_VSP (3Pase on PI(3,4)P2) | 0.15 s−1 | Based on PH-TAPP1 and P4M FRET |
| k_VSP (3Pase on PI(3,4,5)P3) | 44.0 s−1 | Based on PH-Btk and PH-PLCδ1 FRET |
| PH-TAPP1-PI(3,4)P2 | 213 µm−2 | Based on FRET |
| PH-TAPP1 (concentration) | 1 µM | Based on fluorescence intensity |
| KD_TAPP-1 | 0.1 µM | Based on FRET and Dowler et al., 2000 |
| V_0.5_Ci-VSP | 57 mV/95 mV | Villalba-Galea et al., 2013, Fig. 2 C/revised here for Fig. 2 D |
| z_Ci-VSP | 1.56/1.4 | Villalba-Galea et al., 2013, Fig. 2 C/revised here for Fig. 2 D |
The four VSP rate constants k_VSP would be the maximum (fully activated by voltage) values for the red arrows in Fig. 1, chosen to simulate experiments when 50 ng cRNA was injected into the oocyte. These rates were scaled in proportion for each actual injected amount of cRNA, and they were multiplied by a Boltzmann factor between 0 and 1 to give the rates a voltage dependence. Five endogenous lipid kinases and five endogenous lipid phosphatases (black arrows in Fig. 1) kept the same values as in our earlier publication (Keum et al., 2016). Concentrations of membrane molecules are expressed as density per unit area.