Table 1. Statistical summary of the I Ca... | Rockefeller University Press

Table 1.

Statistical summary of the I_Ca density and the Ca²⁺ transient amplitude

	I_Ca density	I_Ca density	Transient amplitude	Transient amplitude	SR Ca²⁺ content
	−40/−25 mV (pA/pF)	−40/0 mV (pA/pF)	−40/−25 mV (ΔF/F₀)	−40/0 mV (ΔF/F₀)	(ΔF/F₀)
WT (N = 5/n = 11)
Control	0.44 ± 0.16	4.83 ± 0.43	0.45 ± 0.09	2.23 ± 0.29	2.84 ± 0.29
Iso	1.98 ± 0.61^{^a}	8.73 ± 0.95^{^a}	1.09 ± 0.23^{^a}	2.47 ± 0.36^{^a}	2.96 ± 0.35
S2030A (N = 5/n = 11)
Control	0.89 ± 0.39	6.87 ± 0.43^{^b}	1.04 ± 0.13^{^b}	2.93 ± 0.16^{^b}	4.46 ± 0.25^{^b}
Iso	2.93 ± 0.93^{^a}	10.82 ± 1.23^{^a}	1.01 ±0.14	2.3 ± 0.19	4.51 ± 0.24

	I_Ca density	I_Ca density	Transient amplitude	Transient amplitude	SR Ca²⁺ content
	−40/−25 mV (pA/pF)	−40/0 mV (pA/pF)	−40/−25 mV (ΔF/F₀)	−40/0 mV (ΔF/F₀)	(ΔF/F₀)
WT (N = 5/n = 11)
Control	0.44 ± 0.16	4.83 ± 0.43	0.45 ± 0.09	2.23 ± 0.29	2.84 ± 0.29
Iso	1.98 ± 0.61^{^a}	8.73 ± 0.95^{^a}	1.09 ± 0.23^{^a}	2.47 ± 0.36^{^a}	2.96 ± 0.35
S2030A (N = 5/n = 11)
Control	0.89 ± 0.39	6.87 ± 0.43^{^b}	1.04 ± 0.13^{^b}	2.93 ± 0.16^{^b}	4.46 ± 0.25^{^b}
Iso	2.93 ± 0.93^{^a}	10.82 ± 1.23^{^a}	1.01 ±0.14	2.3 ± 0.19	4.51 ± 0.24

I_Ca and transients were measured in freshly isolated ventricular cardiomyocytes of WT and S2030A^+/+ knock-in mice. Peak I_Ca was recorded at different membrane potentials (from −40 to 0 mV or −25 to 0 mV) in control conditions (1.8 mM [Ca²⁺]_o) and during 100 nmol/liter Iso application. The protocol shown in Fig. S2 was used to experimentally match the SR Ca²⁺ content between control and Iso in all cells. At matched SR Ca²⁺ load, the Ca²⁺ release in S2030A^+/+ myocytes did not increase during Iso, suggesting a decreased RyRs sensitivity (see Fig. 1). Data are expressed as means ± SEM of n measurements.

P < 0.05 versus control.

P < 0.05 versus WT.

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