| . | n . | κ (mV) . | z(e) . | V1/2 (mV) . | ΔV1/2 (mV) . | ΔΔG0 (kcal/mol) . |
|---|---|---|---|---|---|---|
| WT R230R | 13 | 6.8 | 3.7 | −46.5 ± 3.0 | ||
| R230K | 9 | 7.5 | 3.3 | −95.0 ± 5.1 | 48.5 ± 2.2 | −3.8 ± 0.5 |
| R230H | 8 | 11.7 | 2.1 | −124.6 ± 6.3 | 78.1 ± 2.9 | −3.9 ± 0.6 |
| . | n . | κ (mV) . | z(e) . | V1/2 (mV) . | ΔV1/2 (mV) . | ΔΔG0 (kcal/mol) . |
|---|---|---|---|---|---|---|
| WT R230R | 13 | 6.8 | 3.7 | −46.5 ± 3.0 | ||
| R230K | 9 | 7.5 | 3.3 | −95.0 ± 5.1 | 48.5 ± 2.2 | −3.8 ± 0.5 |
| R230H | 8 | 11.7 | 2.1 | −124.6 ± 6.3 | 78.1 ± 2.9 | −3.9 ± 0.6 |
Summary of V1/2, ΔV1/2, and ΔΔG0 for WT KCNQ3, KCNQ3-R230K, and KCNQ3-R230H substitutions. V1/2 is the midpoint of activation, z is the valence describing the voltage sensitivity of activation, and κ is the slope of the activation curve fitted with the Boltzmann Eq. 1 (see Materials and methods). ΔG0 represents the Gibbs free energy of activation at 0 mV. ΔΔG0 is calculated as [−F(zwtV1/2wt − zmutV1/2mut)] and is expressed in kcal/mol. Values are means ± SEM; n represents the number of cells analyzed.