| Figure | Patch configuration | External | Internal (cytoplasmic) | Vj uncorrecteda |
|---|---|---|---|---|
| Fig. 1 A | Whole cell | B: modified Tyrode’s solution | P: intracellular solution | +13 mV |
| Fig. 1 B | Outside out | B: modified Tyrode’s solution | P: 150 K (phos) | +6 mV |
| Fig. 2 | Inside out | P: 150 Na/1 Ca (HEPES) | B: 150 K and 150 Na (phos) | −6 mV |
| Fig. 3 | Outside out | B: 150 K, 50 K, 20 K, 10 K, 50 K/100 Na, and 20 K/130 Na (phos) | P: 150 K (phos) | 0 |
| Fig. 4 | Cell attached | P: 150 K, 50 K, 20 K, 50 K/100 Na, and 20 K/130 Na (phos) | None | +6, +11, +13, +2, and +1 mV with 150 K, 50 K, 20 K, 50 K/100 Na, and 20 K/130 Na pipette solutions, respectively. |
| B: modified Tyrode’s solution | ||||
| Fig. 5 | Outside out | B: 150 K, 50 K, 20 K, 10 K, 50 K/100 Na, 20 K/100 Na, and 10 K/100 Na (phos) | P: 150 K (phos) | 0 |
| Whole cell | B: modified Tyrode’s solution | P: intracellular solution | +13 mV | |
| Outside out | B: modified Tyrode’s solution | P: 150 K (phos) | +6 mV | |
| Inside out | P: 150 Na/1 Ca (HEPES) | B: 150 K and 150 Na (phos) | −6 mV | |
| Outside out | B: 150 K, 50 K, 20 K, 10 K, 50 K/100 Na, and 20 K/130 Na (phos) | P: 150 K (phos) | 0 | |
| Cell attached | P: 150 K, 50 K, 20 K, 50 K/100 Na, and 20 K/130 Na (phos) | None | +6, +11, +13, +2, and +1 mV with 150 K, 50 K, 20 K, 50 K/100 Na, and 20 K/130 Na pipette solutions, respectively. | |
| B: modified Tyrode’s solution | ||||
| Outside out | B: 150 K, 50 K, 20 K, 10 K, 50 K/100 Na, 20 K/100 Na, and 10 K/100 Na (phos) | P: 150 K (phos) | 0 |
B, bath solution; HEPES, HEPES-buffered solution; P, pipette solution; phos, phosphate-buffered solution.
Vj values (in the bath solution with respect to the pipette solution) were calculated using pCLAMP software (version 10).
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