Table 1. Comparison of uniporter... | Rockefeller University Press

Table 1.

Comparison of uniporter properties characterized using mitoplast patch clamp or Xenopus oocyte recordings

Methods	Mitoplast patch clamp	Xenopus oocyte recordings
Half activating [Ca²⁺]	19 mM	22 mM
Ru360 IC₅₀	1.7 nM	4.2 nM
Divalent ion selectivity	Ca²⁺ ∼ Sr²⁺ >> Mn²⁺ ∼ Ba²⁺ >> Mg²⁺	Ca²⁺ > Sr²⁺ >> Mn²⁺, Ba²⁺, and Mg²⁺
Single-channel amplitude (−80 mV)	<0.5 pA (symmetrical 105 mM Ca²⁺)	∼0.3 pA (100 mM extracellular Ca²⁺ and <1 nM cytoplasmic Ca²⁺)

Methods	Mitoplast patch clamp	Xenopus oocyte recordings
Half activating [Ca²⁺]	19 mM	22 mM
Ru360 IC₅₀	1.7 nM	4.2 nM
Divalent ion selectivity	Ca²⁺ ∼ Sr²⁺ >> Mn²⁺ ∼ Ba²⁺ >> Mg²⁺	Ca²⁺ > Sr²⁺ >> Mn²⁺, Ba²⁺, and Mg²⁺
Single-channel amplitude (−80 mV)	<0.5 pA (symmetrical 105 mM Ca²⁺)	∼0.3 pA (100 mM extracellular Ca²⁺ and <1 nM cytoplasmic Ca²⁺)