Table 2. Parameters of the mathematical... | Rockefeller University Press

Table 2.

Parameters of the mathematical model used to simulate concentration–response data

Parameter	cAMP	cGMP			cAMP
Parameter	cAMP	Wild type	I636A	I636D	Wild type	R591A	T592A	R635A	I636A	I636D	K638A
q (mV)	5	5	5.76	6.72	5	5	5	5.1	5.36	5.05	5
K_d (µM)
Site 1	0.15	0.36	0.24	0.21	0.09	1.64	0.61	0.2	0.47	0.74	0.31
Site 2	1.5	6.13	3.23	1.76	1.39	33.35	11.83	2.19	6.94	17.16	8.00
Site 3	1.5	6.13	3.23	1.76	1.39	33.35	11.83	2.19	6.94	17.16	8.00
Site 4	1.5	6.13	3.23	1.76	1.39	33.35	11.83	2.19	6.94	17.16	8.00
$k_{1}^{c}$	0.75	0.7	0.1	0.06	1.6	1.6	1.6	0.8	1	0.15	1.6
K₁	1.76	1.6	2.3	2.4	1.01	1.01	1.01	1.1	1.5	1.4	1.01
$k_{j 2}^{c}$	9.6	4	0.02	0.08	10	10	10	7	4	0.1	10
K₂	5.36	12	2.8	9	4	4	4	3	4	10	4
$k_{3}^{c}$	43	100	0.02	0.05	80	80	80	40	4	0.1	80
K₃	10.85	14	4	18	38	38	38	8	38	12	38
$k_{4}^{c}$	120	120	0.062	0.05	120	120	120	110	4	0.02	120
K₄	59	33.5	33	33	40	40	40	40	40	40	40

Parameter	cAMP	cGMP			cAMP
Parameter	cAMP	Wild type	I636A	I636D	Wild type	R591A	T592A	R635A	I636A	I636D	K638A
q (mV)	5	5	5.76	6.72	5	5	5	5.1	5.36	5.05	5
K_d (µM)
Site 1	0.15	0.36	0.24	0.21	0.09	1.64	0.61	0.2	0.47	0.74	0.31
Site 2	1.5	6.13	3.23	1.76	1.39	33.35	11.83	2.19	6.94	17.16	8.00
Site 3	1.5	6.13	3.23	1.76	1.39	33.35	11.83	2.19	6.94	17.16	8.00
Site 4	1.5	6.13	3.23	1.76	1.39	33.35	11.83	2.19	6.94	17.16	8.00
$k_{1}^{c}$	0.75	0.7	0.1	0.06	1.6	1.6	1.6	0.8	1	0.15	1.6
K₁	1.76	1.6	2.3	2.4	1.01	1.01	1.01	1.1	1.5	1.4	1.01
$k_{j 2}^{c}$	9.6	4	0.02	0.08	10	10	10	7	4	0.1	10
K₂	5.36	12	2.8	9	4	4	4	3	4	10	4
$k_{3}^{c}$	43	100	0.02	0.05	80	80	80	40	4	0.1	80
K₃	10.85	14	4	18	38	38	38	8	38	12	38
$k_{4}^{c}$	120	120	0.062	0.05	120	120	120	110	4	0.02	120
K₄	59	33.5	33	33	40	40	40	40	40	40	40

The table summarizes the sets of parameters of the mathematical model used for three sets of data for concentration versus the depolarizing shift in the activation curve. cAMP parameters are used to simulate the concentration–response data from Ulens and Siegelbaum (2003), which were used to set up the initial model and are shown in Fig. 1 A along with the simulated curves. cGMP parameters are used to simulate the concentration–response data from Zhou and Siegelbaum (2007). Note that curves for wild-type channel and cGMP were also produced using the gating parameters (⁠ $k_{j}^{c}$ and K_j) for wild type and cAMP. Also, curves for I636A and I636D, with cGMP, were produced using the gating parameters (⁠ $k_{j}^{c}$ and K_j) for the wild-type channel and cGMP. cAMP parameters were used to simulate the concentration–response data from Zhou and Siegelbaum (2007). Note that curves for R635A, I636A, and I636D were also produced using the gating parameters (⁠ $k_{j}^{c}$ and K_j) for the wild-type channel and cAMP.

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