Table 1. Mean values for the parameters... | Rockefeller University Press

Table 1.

Mean values for the parameters obtained from fitting a Boltzmann function to the FRET ratio (R/R₀) versus voltage data obtained with the different Mermaid constructs

Main subcellular distribution	Mermaid T-tubule (and SR?)	Rv-Mermaid T-tubule (and SR?)	Rv-Mermaid ^D129E/Y235R T-tubule and SR^{^a}	T306-Rv-Mermaid Junctional SR	T306-Rv-Mermaid ^D129E/Y235R Junctional SR
n	6	6	6	6	13
Max	0.17 ± 0.02	0.16 ± 0.02	0.17 ± 0.02	−0.075 ± 0.01	−0.06 ± 0.01
Min	0.99 ± 0.01	0.95 ± 0.01	0.93 ± 0.01	1	1
V_0.5 (mV)	−37.7 ± 7.1	−69.0 ± 4.1	5.45 ± 3.4	−26.4 ± 1.2	−26.2 ± 1.9
k (mV)	14.1 ± 0.46	14.9 ± 1.1	11.4 ± 0.5	5.5 ± 0.9	2.8 ± 0.9

Main subcellular distribution	Mermaid T-tubule (and SR?)	Rv-Mermaid T-tubule (and SR?)	Rv-Mermaid ^D129E/Y235R T-tubule and SR^{^a}	T306-Rv-Mermaid Junctional SR	T306-Rv-Mermaid ^D129E/Y235R Junctional SR
n	6	6	6	6	13
Max	0.17 ± 0.02	0.16 ± 0.02	0.17 ± 0.02	−0.075 ± 0.01	−0.06 ± 0.01
Min	0.99 ± 0.01	0.95 ± 0.01	0.93 ± 0.01	1	1
V_0.5 (mV)	−37.7 ± 7.1	−69.0 ± 4.1	5.45 ± 3.4	−26.4 ± 1.2	−26.2 ± 1.9
k (mV)	14.1 ± 0.46	14.9 ± 1.1	11.4 ± 0.5	5.5 ± 0.9	2.8 ± 0.9

Individual sets of data points from each fiber were fitted with the following expression: Max/{1 + exp[(V_0.5 − V)/k]} + Min. Data are mean ± SEM.

The voltage dependence was established from signals elicited by pulses from 0 mV.

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