Table 1.
Whole-cell recordings of fertilization using TMEM16A inhibitors
Resting potential (mV)Fertilization potential (mV)Sperm to depolarization (min)Depolarization rate (mV/ms)n
Control (MR/5) −19.2 ± 1.0 3.7 ± 2.3 4.9 ± 0.7 9.0 ± 3.4 30 
10 µM MONNA −12.8 ± 0.8 n/a n/a n/a 
1 µM Ani9 −14.7 ± 2.5 9.3 ± 5.2 5.4 ± 0.8 1.21 ± 1.15 
Resting potential (mV)Fertilization potential (mV)Sperm to depolarization (min)Depolarization rate (mV/ms)n
Control (MR/5) −19.2 ± 1.0 3.7 ± 2.3 4.9 ± 0.7 9.0 ± 3.4 30 
10 µM MONNA −12.8 ± 0.8 n/a n/a n/a 
1 µM Ani9 −14.7 ± 2.5 9.3 ± 5.2 5.4 ± 0.8 1.21 ± 1.15 

Mean ± SEM values for each fertilization condition are shown. n reports the number of recordings from individual eggs. In conditions where fertilization failed to evoke any depolarization, n/a reflects that were no postdepolarization data to quantify postfertilization metrics.

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