| Construct . | V½ (mV) . | s (mV) . | n . |
|---|---|---|---|
| WT | ‒23.3 ± 1.1 | 6.6 ± 0.2 | 15 |
| M356R/A359R/R362R (=3R) | +24.2 ± 0.9 | 9.3 ± 0.5 | 7 |
| Y323C | ‒5.7 ± 1.8 | 9.6 ± 1.1 | 8 |
| F324C | ‒9.5 ± 1.4 | 13.4 ± 0.6 | 10 |
| T326C | ‒10.0 ± 2.4 | 13.1 ± 0.9 | 9 |
| Y323C/F324C/T326C | +17.9 ± 2.5 | 13.1 ± 0.4 | 7 |
| R365C | +19.2 ± 1.0 | 8.9 ± 0.6 | 7 |
| Construct . | V½ (mV) . | s (mV) . | n . |
|---|---|---|---|
| WT | ‒23.3 ± 1.1 | 6.6 ± 0.2 | 15 |
| M356R/A359R/R362R (=3R) | +24.2 ± 0.9 | 9.3 ± 0.5 | 7 |
| Y323C | ‒5.7 ± 1.8 | 9.6 ± 1.1 | 8 |
| F324C | ‒9.5 ± 1.4 | 13.4 ± 0.6 | 10 |
| T326C | ‒10.0 ± 2.4 | 13.1 ± 0.9 | 9 |
| Y323C/F324C/T326C | +17.9 ± 2.5 | 13.1 ± 0.4 | 7 |
| R365C | +19.2 ± 1.0 | 8.9 ± 0.6 | 7 |
V½ and s were determined using Eq. 2 in Materials and methods raised to the power of 1. Data shown as mean ± SEM.