Table 1.
Biophysical properties of the fertilization-evoked depolarizations
Resting potentialFertilization potentialSperm addition to depolarization timeDepolarization raten
 mV mV min mV/ms  
Control (MR/5) −17.9 ± 0.9 −2.6 ± 1.2 5.3 ± 0.5 3.9 ± 0.8 31 
10 µM GdCl3 −17.0 ± 2.3 −2.6 ± 0.7 3.5 ± 0.8 10.6 ± 7.9 8 
20 µM SK&F-96365 −13.6 ± 2.0 −6.4 ± 1.9 3.8 ± 1.0 6.1 ± 3.6 6 
500 nM Xestospongin C −11.0 ± 1.4 — — — 5 
2% DMSO −21.5 ± 4.2 −6.6 ± 2.3 8.0 ± 1.4 5.8 ± 3.4 5 
100 µM 2-APB −19.6 ± 1.4 −4.8 ± 2.0 6.6 ± 1.8 0.6 ± 0.3 8 
1 µM U73122 −22.1 ± 4.1 — — — 7 
1 µM U73343 −20.8 ± 2.3 −3.7 ± 1.2 1.9 ± 0.4 14.9 ± 6.6 8 
Resting potentialFertilization potentialSperm addition to depolarization timeDepolarization raten
 mV mV min mV/ms  
Control (MR/5) −17.9 ± 0.9 −2.6 ± 1.2 5.3 ± 0.5 3.9 ± 0.8 31 
10 µM GdCl3 −17.0 ± 2.3 −2.6 ± 0.7 3.5 ± 0.8 10.6 ± 7.9 8 
20 µM SK&F-96365 −13.6 ± 2.0 −6.4 ± 1.9 3.8 ± 1.0 6.1 ± 3.6 6 
500 nM Xestospongin C −11.0 ± 1.4 — — — 5 
2% DMSO −21.5 ± 4.2 −6.6 ± 2.3 8.0 ± 1.4 5.8 ± 3.4 5 
100 µM 2-APB −19.6 ± 1.4 −4.8 ± 2.0 6.6 ± 1.8 0.6 ± 0.3 8 
1 µM U73122 −22.1 ± 4.1 — — — 7 
1 µM U73343 −20.8 ± 2.3 −3.7 ± 1.2 1.9 ± 0.4 14.9 ± 6.6 8 

Mean ± SEM for the indicated measurement before, during, and after the fertilization-signaled depolarization.

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