Table 1. Biophysical properties of the... | Rockefeller University Press

Table 1.

Biophysical properties of the fertilization-evoked depolarizations

	Resting potential	Fertilization potential	Sperm addition to depolarization time	Depolarization rate	n
	mV	mV	min	mV/ms
Control (MR/5)	−17.9 ± 0.9	−2.6 ± 1.2	5.3 ± 0.5	3.9 ± 0.8	31
10 µM GdCl₃	−17.0 ± 2.3	−2.6 ± 0.7	3.5 ± 0.8	10.6 ± 7.9	8
20 µM SK&F-96365	−13.6 ± 2.0	−6.4 ± 1.9	3.8 ± 1.0	6.1 ± 3.6	6
500 nM Xestospongin C	−11.0 ± 1.4	—	—	—	5
2% DMSO	−21.5 ± 4.2	−6.6 ± 2.3	8.0 ± 1.4	5.8 ± 3.4	5
100 µM 2-APB	−19.6 ± 1.4	−4.8 ± 2.0	6.6 ± 1.8	0.6 ± 0.3	8
1 µM U73122	−22.1 ± 4.1	—	—	—	7
1 µM U73343	−20.8 ± 2.3	−3.7 ± 1.2	1.9 ± 0.4	14.9 ± 6.6	8

	Resting potential	Fertilization potential	Sperm addition to depolarization time	Depolarization rate	n
	mV	mV	min	mV/ms
Control (MR/5)	−17.9 ± 0.9	−2.6 ± 1.2	5.3 ± 0.5	3.9 ± 0.8	31
10 µM GdCl₃	−17.0 ± 2.3	−2.6 ± 0.7	3.5 ± 0.8	10.6 ± 7.9	8
20 µM SK&F-96365	−13.6 ± 2.0	−6.4 ± 1.9	3.8 ± 1.0	6.1 ± 3.6	6
500 nM Xestospongin C	−11.0 ± 1.4	—	—	—	5
2% DMSO	−21.5 ± 4.2	−6.6 ± 2.3	8.0 ± 1.4	5.8 ± 3.4	5
100 µM 2-APB	−19.6 ± 1.4	−4.8 ± 2.0	6.6 ± 1.8	0.6 ± 0.3	8
1 µM U73122	−22.1 ± 4.1	—	—	—	7
1 µM U73343	−20.8 ± 2.3	−3.7 ± 1.2	1.9 ± 0.4	14.9 ± 6.6	8

Mean ± SEM for the indicated measurement before, during, and after the fertilization-signaled depolarization.