Table 2. Effects of mutations on pIC 50... | Rockefeller University Press

Table 2.

Effects of mutations on pIC₅₀ values for Zn²⁺ modulation of Hv1 gating

Insertion	Mutant 2	Mean pIC₅₀ Mutant 2 (pIC_{50_2})	Mutant 1	Mean pIC₅₀ Mutant 1 (pIC_{50_1})	Mean ΔpIC₅₀ = (pIC_{50_1} - pIC_{50_2})	t value: t test	p-value: t test
E119H (E^1.58H)	E119H-H140A-H193A	−5.15	H140A-H193A	−2.76	2.39	−25.1	1.8 × 10⁻⁰⁶*
E119H (E^1.58H)	E119H-H140A-D185H-H193A	−4.90	H140A-D185H-H193A	−3.15	1.75	−9.7	0.0005*
E119H (E^1.58H)	E119H-H140A	−6.23	H140A	−4.41	1.81	−16.9	7.0 × 10⁻⁰⁵*
D130H (D^1.69H)	D130H-H193A	−5.51	H193A	−4.06	1.44	−6.6	0.003*
E119H (E^1.58H)	E119H-H193A	−5.45	H193A	−4.06	1.38	−10.6	0.0004*
E119H (E^1.58H)	E119H	−6.22	WT	−5.44	0.77	−5.7	0.004*
R205H (R1H/ R^4.47H)	H140A-D185H-H193A-R205H	−3.68	H140A-D185H-H193A	−3.15	0.54	−4.2	0.013*
D185H (D^3.61H)	H140A-D185H	−4.96	H140A	−4.41	0.54	−5.3	0.005*
D185H (D^3.61H)	D185H-H193A	−4.61	H193A	−4.06	0.52	−2.0	0.11
D123H (D^1.62H)	D123H-H193A	−4.54	H193A	−4.06	0.47	−2.2	0.073
D185H (D^3.61H)	H140A-D185H-H193A	−3.15	H140A-H193A	−2.76	0.38	−3.6	0.015*
D123H (D^1.62H)	D123H-H140A	−4.78	H140A	−4.41	0.36	−2.5	0.041*
D130H (D^1.69H)	D130H	−5.67	WT	−5.44	0.22	−0.80	0.45
D123H (D^1.62H)	D123H	−5.56	WT	−5.44	0.11	−0.36	0.73
D130H (D^1.69H)	D130H-H140A	−4.42	H140A	−4.41	0.01	−0.05	0.95
D112N (D^1.51N)	D112N	−5.03	WT	−5.06	−0.03	0.16	0.87
H193A (H^3.71A)	D130H-H193A	−5.51	D130H	−5.67	−0.16	0.52	0.62
D185H (D^3.61H)	E119H- H140A-D185H- H193A	−4.90	E119H-H140A-H193A	−5.15	−0.25	1.4	0.21
E119A (E^1.58A)	E119A	−5.16	WT	−5.44	−0.28	1.6	0.17
H140A (H^2.40A)	H140A-D185H	−4.96	D185H	−5.38	−0.42	3.6	0.021*
E119A (E^1.58A)	E119A-H193A	−3.56	H193A	−4.06	−0.50	4.6	0.009*
H193A (H^3.71A)	D185H-H193A	−4.54	D185H	−5.38	−0.84	3.5	0.023*
E196H (E^4.38H)	E196H	−4.63	WT	−5.06	−0.43	−1.1	0.3
H140A (H^2.40A)	H140A	−4.41	WT	−5.44	−1.02	7.0	0.002*
E153A (E^2.53A)	E153A	−3.77	WT	−5.06	−1.29	3.3	0.02*
H140A (H^2.40A)	H140A-H193A	−2.76	H193A	−4.06	−1.30	24.5	2.1 × 10⁻⁶*
H193A (H^3.71A)	H193A	−4.06	WT	−5.44	−1.37	9.1	0.0007*
H193A (H^3.71A)	H140A-H193A	−2.76	H140A	−4.41	−1.65	24.5	2.1 × 10⁻⁶*
H193A (H^3.71A)	E119A-H193A	−3.56	E119A	−5.16	−1.60	10.6	0.0001*
D112H (D^1.51H)	D112H	−2.96	WT	−5.06	−2.1	10.7	0.001*
H140A-H193A (H^2.40A-(H^3.71A)	H140A-H193A	−2.76	WT	−5.44	−2.68	23.1	2.7 × 10⁻⁶*

Insertion	Mutant 2	Mean pIC₅₀ Mutant 2 (pIC_{50_2})	Mutant 1	Mean pIC₅₀ Mutant 1 (pIC_{50_1})	Mean ΔpIC₅₀ = (pIC_{50_1} - pIC_{50_2})	t value: t test	p-value: t test
E119H (E^1.58H)	E119H-H140A-H193A	−5.15	H140A-H193A	−2.76	2.39	−25.1	1.8 × 10⁻⁰⁶*
E119H (E^1.58H)	E119H-H140A-D185H-H193A	−4.90	H140A-D185H-H193A	−3.15	1.75	−9.7	0.0005*
E119H (E^1.58H)	E119H-H140A	−6.23	H140A	−4.41	1.81	−16.9	7.0 × 10⁻⁰⁵*
D130H (D^1.69H)	D130H-H193A	−5.51	H193A	−4.06	1.44	−6.6	0.003*
E119H (E^1.58H)	E119H-H193A	−5.45	H193A	−4.06	1.38	−10.6	0.0004*
E119H (E^1.58H)	E119H	−6.22	WT	−5.44	0.77	−5.7	0.004*
R205H (R1H/ R^4.47H)	H140A-D185H-H193A-R205H	−3.68	H140A-D185H-H193A	−3.15	0.54	−4.2	0.013*
D185H (D^3.61H)	H140A-D185H	−4.96	H140A	−4.41	0.54	−5.3	0.005*
D185H (D^3.61H)	D185H-H193A	−4.61	H193A	−4.06	0.52	−2.0	0.11
D123H (D^1.62H)	D123H-H193A	−4.54	H193A	−4.06	0.47	−2.2	0.073
D185H (D^3.61H)	H140A-D185H-H193A	−3.15	H140A-H193A	−2.76	0.38	−3.6	0.015*
D123H (D^1.62H)	D123H-H140A	−4.78	H140A	−4.41	0.36	−2.5	0.041*
D130H (D^1.69H)	D130H	−5.67	WT	−5.44	0.22	−0.80	0.45
D123H (D^1.62H)	D123H	−5.56	WT	−5.44	0.11	−0.36	0.73
D130H (D^1.69H)	D130H-H140A	−4.42	H140A	−4.41	0.01	−0.05	0.95
D112N (D^1.51N)	D112N	−5.03	WT	−5.06	−0.03	0.16	0.87
H193A (H^3.71A)	D130H-H193A	−5.51	D130H	−5.67	−0.16	0.52	0.62
D185H (D^3.61H)	E119H- H140A-D185H- H193A	−4.90	E119H-H140A-H193A	−5.15	−0.25	1.4	0.21
E119A (E^1.58A)	E119A	−5.16	WT	−5.44	−0.28	1.6	0.17
H140A (H^2.40A)	H140A-D185H	−4.96	D185H	−5.38	−0.42	3.6	0.021*
E119A (E^1.58A)	E119A-H193A	−3.56	H193A	−4.06	−0.50	4.6	0.009*
H193A (H^3.71A)	D185H-H193A	−4.54	D185H	−5.38	−0.84	3.5	0.023*
E196H (E^4.38H)	E196H	−4.63	WT	−5.06	−0.43	−1.1	0.3
H140A (H^2.40A)	H140A	−4.41	WT	−5.44	−1.02	7.0	0.002*
E153A (E^2.53A)	E153A	−3.77	WT	−5.06	−1.29	3.3	0.02*
H140A (H^2.40A)	H140A-H193A	−2.76	H193A	−4.06	−1.30	24.5	2.1 × 10⁻⁶*
H193A (H^3.71A)	H193A	−4.06	WT	−5.44	−1.37	9.1	0.0007*
H193A (H^3.71A)	H140A-H193A	−2.76	H140A	−4.41	−1.65	24.5	2.1 × 10⁻⁶*
H193A (H^3.71A)	E119A-H193A	−3.56	E119A	−5.16	−1.60	10.6	0.0001*
D112H (D^1.51H)	D112H	−2.96	WT	−5.06	−2.1	10.7	0.001*
H140A-H193A (H^2.40A-(H^3.71A)	H140A-H193A	−2.76	WT	−5.44	−2.68	23.1	2.7 × 10⁻⁶*

Data are ranked by ΔpIC₅₀; positive values indicate an increase in Zn²⁺ potency whereas negative values reflect a loss in potency. Statistically significant (*, P < 0.05 by Student’s unpaired t test) differences in ΔpIC₅₀ values are indicated by asterisks.

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