Table 1. Effects of Hv1 mutations on V... | Rockefeller University Press

Table 1.

Effects of Hv1 mutations on V_0.5 and Zn²⁺ potency

Mutations	V_STEP	Fit V_0.5	SD	n	Mean τ_ACT @ V_STEP	SEM	n	Mean IC₅₀ @ V_STEP	SEM	pIC₅₀	n	Hill value	SEM
	mV	mV			ms			μM
WT	100	62.4	2.9	5	1,055	262	5	3.8	1.7	−5.44	3	0.81	0.20
H140A (H^2.40A)	100	80.1	3.7	3	2,008	1,016	3	38.9	9.4	−4.41	3	0.67	0.07
H193A (H^3.71A)	100	58.3	1.7	3	759	261	3	87.8	23.8	−4.06	3	1.04	0.02
H140A-H193A (H^2.40A-H^3.71A)	100	79.1	5.7	4	1,802	235	4	1,754.4	272.7	−2.76	4	1.57	0.13
E119A (E^1.58A)	120	98.5	17.8	4	1,666	553	4	7.55	4.0	−5.16	4	0.80	0.14
E119A-H193A (E^1.58A-H^3.71A)	100	84.5	2.4	4	1,095	556	4	281.79	79.8	−3.56	3	0.78	0.09
E119H (E^1.58H)	100	90.4	2.8	5	625	170	5	0.606	0.06	−6.22	3	1.08	0.40
E119H-H140A (E^1.58H-H^2.40A)	120	99.1	6.1	6	1,598	356	6	0.60	0.17	−6.23	4	2.09	0.90
E119H-H193A (E^1.58H-H^3.71A)	100	97.8	2.9	3	1,273	493	3	3.7	1.5	−5.45	3	0.86	0.15
E119H-H140A-H193A (E^1.58H-H^2.40A-H^3.71A)	60	67.9	2.0	4	429	75	4	7.3	3.2	−5.15	3	0.84	0.23
D123H (D^1.62H)	80	94.6	1.9	4	857	303	4	4.3	4.9	−5.56	3	0.95	0.20
D123H-H140A (D^1.62H-H^2.40A)	80	95.8	2.2	5	1,115	254	5	18.0	8.6	−4.78	5	0.58	0.08
D123H-H193A (D^1.62H-H^3.71A)	80	101.6	1.7	4	760	201	4	36.0	28.1	−4.54	4	1.06	0.14
D130H (D^1.69H)	120	87.4	2.0	4	1,788	948	4	3.0	2.9	−5.67	4	0.95	0.35
D130H-H140A (D^1.69H-H^2.40A)	80	92.0	3.7	5	1,111	413	5	41.5	19.7	−4.42	4	0.58	0.16
D130H-H193A (D^1.69H-H^3.71A)	120	87.3	2.8	4	1,217	628	4	3.7	2.7	−5.51	3	0.91	0.17
D185H (D^3.61H)	120	130.0	4.3	3	886	153	3	4.2	1.5	−5.38	3	0.77	0.15
H140A-D185H (H^2.40A-D^3.61H)	120	74.2	4.5	4	1,169	386	4	11.2	3.7	−4.96	3	0.81	0.35
D185H-H193A (D^3.61H-H^3.71A)	80	95.8	7.4	3	1,328	492	3	37.9	35.6	−4.54	3	0.60	0.09
H140A-D185H-H193A (H^2.40A-D^3.61H-H^3.71A)	100	63.9	2.7	3	1,050	277	3	762.3	308.1	−3.15	3	1.05	0.10
E119H-H140A- D185H-H193A (E^1.58H-H^2.40A-D^3.61H-H^3.71A)	120	107.4	2.8	3	308	3	3	13.8	8.0	−4.90	3	0.72	0.10
D185A-H193A (D^3.61A-H^3.71A)	140	104.8	1.8	4	584	113	4	135.1	40.7	−3.88	4	0.62	0.19
E196H (E^4.38H)	100	45.0	5.8	4	579	113	4	37.2	46.8	−4.63	4	0.63	0.15
E153A (E^2.53A)	40	5.4	2.3	4	306	82	4	241.2	50.1	−3.77	4	0.87	0.16
D112N (D^1.51N)	140	120.3	4.8	4	–	–	–	9.5	2.8	−5.03	3	0.76	0.28
D112H (D^1.51N)	130	117.4	4.9	3	–	–	–	1,139.3	429.4	−2.96	3	0.56	0.03

Mutations	V_STEP	Fit V_0.5	SD	n	Mean τ_ACT @ V_STEP	SEM	n	Mean IC₅₀ @ V_STEP	SEM	pIC₅₀	n	Hill value	SEM
	mV	mV			ms			μM
WT	100	62.4	2.9	5	1,055	262	5	3.8	1.7	−5.44	3	0.81	0.20
H140A (H^2.40A)	100	80.1	3.7	3	2,008	1,016	3	38.9	9.4	−4.41	3	0.67	0.07
H193A (H^3.71A)	100	58.3	1.7	3	759	261	3	87.8	23.8	−4.06	3	1.04	0.02
H140A-H193A (H^2.40A-H^3.71A)	100	79.1	5.7	4	1,802	235	4	1,754.4	272.7	−2.76	4	1.57	0.13
E119A (E^1.58A)	120	98.5	17.8	4	1,666	553	4	7.55	4.0	−5.16	4	0.80	0.14
E119A-H193A (E^1.58A-H^3.71A)	100	84.5	2.4	4	1,095	556	4	281.79	79.8	−3.56	3	0.78	0.09
E119H (E^1.58H)	100	90.4	2.8	5	625	170	5	0.606	0.06	−6.22	3	1.08	0.40
E119H-H140A (E^1.58H-H^2.40A)	120	99.1	6.1	6	1,598	356	6	0.60	0.17	−6.23	4	2.09	0.90
E119H-H193A (E^1.58H-H^3.71A)	100	97.8	2.9	3	1,273	493	3	3.7	1.5	−5.45	3	0.86	0.15
E119H-H140A-H193A (E^1.58H-H^2.40A-H^3.71A)	60	67.9	2.0	4	429	75	4	7.3	3.2	−5.15	3	0.84	0.23
D123H (D^1.62H)	80	94.6	1.9	4	857	303	4	4.3	4.9	−5.56	3	0.95	0.20
D123H-H140A (D^1.62H-H^2.40A)	80	95.8	2.2	5	1,115	254	5	18.0	8.6	−4.78	5	0.58	0.08
D123H-H193A (D^1.62H-H^3.71A)	80	101.6	1.7	4	760	201	4	36.0	28.1	−4.54	4	1.06	0.14
D130H (D^1.69H)	120	87.4	2.0	4	1,788	948	4	3.0	2.9	−5.67	4	0.95	0.35
D130H-H140A (D^1.69H-H^2.40A)	80	92.0	3.7	5	1,111	413	5	41.5	19.7	−4.42	4	0.58	0.16
D130H-H193A (D^1.69H-H^3.71A)	120	87.3	2.8	4	1,217	628	4	3.7	2.7	−5.51	3	0.91	0.17
D185H (D^3.61H)	120	130.0	4.3	3	886	153	3	4.2	1.5	−5.38	3	0.77	0.15
H140A-D185H (H^2.40A-D^3.61H)	120	74.2	4.5	4	1,169	386	4	11.2	3.7	−4.96	3	0.81	0.35
D185H-H193A (D^3.61H-H^3.71A)	80	95.8	7.4	3	1,328	492	3	37.9	35.6	−4.54	3	0.60	0.09
H140A-D185H-H193A (H^2.40A-D^3.61H-H^3.71A)	100	63.9	2.7	3	1,050	277	3	762.3	308.1	−3.15	3	1.05	0.10
E119H-H140A- D185H-H193A (E^1.58H-H^2.40A-D^3.61H-H^3.71A)	120	107.4	2.8	3	308	3	3	13.8	8.0	−4.90	3	0.72	0.10
D185A-H193A (D^3.61A-H^3.71A)	140	104.8	1.8	4	584	113	4	135.1	40.7	−3.88	4	0.62	0.19
E196H (E^4.38H)	100	45.0	5.8	4	579	113	4	37.2	46.8	−4.63	4	0.63	0.15
E153A (E^2.53A)	40	5.4	2.3	4	306	82	4	241.2	50.1	−3.77	4	0.87	0.16
D112N (D^1.51N)	140	120.3	4.8	4	–	–	–	9.5	2.8	−5.03	3	0.76	0.28
D112H (D^1.51N)	130	117.4	4.9	3	–	–	–	1,139.3	429.4	−2.96	3	0.56	0.03

Measured V_0.5, τ_ACT, and Zn²⁺ IC₅₀ and pIC₅₀ values for each Hv1 mutant construct in this study are shown. Note that on average, V_STEP = V_0.5 + 12.7 mV (except for E196H, V_STEP >26 mV or ≤3 mV different from V_0.5), indicating that τ_ACT and Zn²⁺ potency (IC₅₀) are determined at similar apparent P_OPEN values. Values for D112N/H were determined from tail current.

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