Table 2.
Mean open durations for different agonists after oxidation and reduction of αD97C/αK125C receptors
Agonist/receptor (number of transfections)Treatment (number of patches)mOpen1Area1mOpen2Area2mOpen3Area3
Cho/wild type (3) Control (4)   2.3 ± 0.3 × 10−4 1.0 ± 0.03   
 H2O2 (6)   2.2 ± 0.2 × 10−4 1.0 ± 0.03   
 DTT (4)   1.9 ± 0.2 × 10−4 1.0 ± 0.05   
Cho/αD97C/αK125C (6) Control (4) 3.3 ± 1.0 × 10−5 0.3 ± 0.04 6.8 ± 0.4 × 10−4 0.6 ± 0.07 3.3 ± 0.2 × 10−3 0.05 ± 0.01 
 H2O2 (6) 4.1 ± 0.4 × 10−5 0.8 ± 0.03 1.9 ± 0.8 × 10−4 0.2 ± 0.09   
 DTT (6) 3.7 ± 0.7 × 10−5 0.3 ± 0.06 7.3 ± 0.3 × 10−4 0.6 ± 0.03 4.4 ± 0.1 × 10−3 0.1 ± 0.02 
Cho/αY125C (1) Control (4) 2.4 ± 0.3 × 10−5 0.4 ± 0.08 2.7 ± 0.2 × 10−4 0.6 ± 0.08   
 H2O2 (6) 3.1 ± 0.5 × 10−5 0.4 ± 0.04 2.7 ± 0.3 × 10−4 0.6 ± 0.04   
 DTT (4) 3.1 ± 0.6 × 10−5 0.3 ± 0.06 2.6 ± 0.4 × 10−4 0.6 ± 0.06   
ACh/αD97C/αK125C (3) Control (6) 3.9 ± 0.2 × 10−5 0.3 ± 0.02 1.2 ± 0.8 × 10−4 0.3 ± 0.02 3.1 ± 0.10 × 10−3 0.4 ± 0.05 
 H2O2 (8) 2.7 ± 0.9 × 10−5 0.2 ± 0.05 1.9 ± 1.0 × 10−4 0.3 ± 0.04 3.2 ± 0.7 × 10−3 0.5 ± 0.04 
 DTT (6) 1.7 ± 0.5 × 10−5 0.1 ± 0.03 2.2 ± 0.2 × 10−4 0.2 ± 0.02 6.0 ± 0.1 × 10−3 0.6 ± 0.05 
ACh/αY125C (3) Control (4) 5.8 ± 0.7 × 10−5 0.3 ± 0.06 4.5 ± 0.3 × 10−4 0.7 ± 0.06   
 H2O2 (4) 6.0 ± 0.1 × 10−5 0.3 ± 0.05 4.8 ± 0.2 × 10−4 0.7 ± 0.05   
 DTT (4) 5.4 ± 0.1 × 10−5 0.3 ± 0.04 4.6 ± 0.5 × 10−4 0.7 ± 0.06   
SubCho/αD97C/αK125C (6) Control (4) 8.0 ± 0.7 × 10−5 0.6 ± 0.02 5.1 ± 0.4 × 10−4 0.4 ± 0.02 1.4 ± 0.3 × 10−2 0.02 ± 0.01 
 H2O2 (6) 8.3 ± 0.2 × 10−5 0.6 ± 0.03 7.4 ± 0.9 × 10−4 0.4 ± 0.01 1.2 ± 0.5 × 10−2 0.02 ± 0.01 
 DTT (4) 7.1 ± 0.2 × 10−5 0.5 ± 0.02 5.7 ± 0.4 × 10−4 0.3 ± 0.03 1.3 ± 0.2 × 10−2 0.1 ± 0.01 
CCh/αD97C/αK25C (3) Control (4)   1.6 ± 0.3 × 10−4 0.2 ± 0.07 3.0 ± 0.8 × 10−3 0.8 ± 0.02 
 H2O2 (4)   1.4 ± 0.7 × 10−4 0.1 ± 0.04 2.4 ± 0.2 × 10−3 0.9 ± 0.03 
 DTT (4)   2.2 ± 0.2 × 10−4 0.4 ± 0.03 2.3 ± 0.2 × 10−3 0.6 ± 0.03 
PIP/αD97C/αK125C (3) Control (4)   2.3 ± 0.4 × 10−4 0.6 ± 0.05 3.4 ± 0.2 × 10−3 0.4 ± 0.03 
 H2O2 (4)   2.1 ± 0.2 × 10−4 0.4 ± 0.08 2.7 ± 0.4 × 10−3 0.6 ± 0.10 
 DTT (4)   2.5 ± 0.7 × 10−4 0.4 ± 0.03 2.6 ± 0.3 × 10−3 0.6 ± 0.03 
TMA/αD97C/αK125C (3) Control (4)   3.3 ± 0.3 × 10−4 0.2 ± 0.03 1.0 ± 0.2 × 10−3 0.8 ± 0.10 
 H2O2 (4)   1.2 ± 0.3 × 10−4 0.4 ± 0.02 1.1 ± 0.1 × 10−3 0.6 ± 0.05 
 DTT (4)   2.2 ± 0.2 × 10−4 0.4 ± 0.07 9.9 ± 0.1 × 10−4 0.6 ± 0.07 
Agonist/receptor (number of transfections)Treatment (number of patches)mOpen1Area1mOpen2Area2mOpen3Area3
Cho/wild type (3) Control (4)   2.3 ± 0.3 × 10−4 1.0 ± 0.03   
 H2O2 (6)   2.2 ± 0.2 × 10−4 1.0 ± 0.03   
 DTT (4)   1.9 ± 0.2 × 10−4 1.0 ± 0.05   
Cho/αD97C/αK125C (6) Control (4) 3.3 ± 1.0 × 10−5 0.3 ± 0.04 6.8 ± 0.4 × 10−4 0.6 ± 0.07 3.3 ± 0.2 × 10−3 0.05 ± 0.01 
 H2O2 (6) 4.1 ± 0.4 × 10−5 0.8 ± 0.03 1.9 ± 0.8 × 10−4 0.2 ± 0.09   
 DTT (6) 3.7 ± 0.7 × 10−5 0.3 ± 0.06 7.3 ± 0.3 × 10−4 0.6 ± 0.03 4.4 ± 0.1 × 10−3 0.1 ± 0.02 
Cho/αY125C (1) Control (4) 2.4 ± 0.3 × 10−5 0.4 ± 0.08 2.7 ± 0.2 × 10−4 0.6 ± 0.08   
 H2O2 (6) 3.1 ± 0.5 × 10−5 0.4 ± 0.04 2.7 ± 0.3 × 10−4 0.6 ± 0.04   
 DTT (4) 3.1 ± 0.6 × 10−5 0.3 ± 0.06 2.6 ± 0.4 × 10−4 0.6 ± 0.06   
ACh/αD97C/αK125C (3) Control (6) 3.9 ± 0.2 × 10−5 0.3 ± 0.02 1.2 ± 0.8 × 10−4 0.3 ± 0.02 3.1 ± 0.10 × 10−3 0.4 ± 0.05 
 H2O2 (8) 2.7 ± 0.9 × 10−5 0.2 ± 0.05 1.9 ± 1.0 × 10−4 0.3 ± 0.04 3.2 ± 0.7 × 10−3 0.5 ± 0.04 
 DTT (6) 1.7 ± 0.5 × 10−5 0.1 ± 0.03 2.2 ± 0.2 × 10−4 0.2 ± 0.02 6.0 ± 0.1 × 10−3 0.6 ± 0.05 
ACh/αY125C (3) Control (4) 5.8 ± 0.7 × 10−5 0.3 ± 0.06 4.5 ± 0.3 × 10−4 0.7 ± 0.06   
 H2O2 (4) 6.0 ± 0.1 × 10−5 0.3 ± 0.05 4.8 ± 0.2 × 10−4 0.7 ± 0.05   
 DTT (4) 5.4 ± 0.1 × 10−5 0.3 ± 0.04 4.6 ± 0.5 × 10−4 0.7 ± 0.06   
SubCho/αD97C/αK125C (6) Control (4) 8.0 ± 0.7 × 10−5 0.6 ± 0.02 5.1 ± 0.4 × 10−4 0.4 ± 0.02 1.4 ± 0.3 × 10−2 0.02 ± 0.01 
 H2O2 (6) 8.3 ± 0.2 × 10−5 0.6 ± 0.03 7.4 ± 0.9 × 10−4 0.4 ± 0.01 1.2 ± 0.5 × 10−2 0.02 ± 0.01 
 DTT (4) 7.1 ± 0.2 × 10−5 0.5 ± 0.02 5.7 ± 0.4 × 10−4 0.3 ± 0.03 1.3 ± 0.2 × 10−2 0.1 ± 0.01 
CCh/αD97C/αK25C (3) Control (4)   1.6 ± 0.3 × 10−4 0.2 ± 0.07 3.0 ± 0.8 × 10−3 0.8 ± 0.02 
 H2O2 (4)   1.4 ± 0.7 × 10−4 0.1 ± 0.04 2.4 ± 0.2 × 10−3 0.9 ± 0.03 
 DTT (4)   2.2 ± 0.2 × 10−4 0.4 ± 0.03 2.3 ± 0.2 × 10−3 0.6 ± 0.03 
PIP/αD97C/αK125C (3) Control (4)   2.3 ± 0.4 × 10−4 0.6 ± 0.05 3.4 ± 0.2 × 10−3 0.4 ± 0.03 
 H2O2 (4)   2.1 ± 0.2 × 10−4 0.4 ± 0.08 2.7 ± 0.4 × 10−3 0.6 ± 0.10 
 DTT (4)   2.5 ± 0.7 × 10−4 0.4 ± 0.03 2.6 ± 0.3 × 10−3 0.6 ± 0.03 
TMA/αD97C/αK125C (3) Control (4)   3.3 ± 0.3 × 10−4 0.2 ± 0.03 1.0 ± 0.2 × 10−3 0.8 ± 0.10 
 H2O2 (4)   1.2 ± 0.3 × 10−4 0.4 ± 0.02 1.1 ± 0.1 × 10−3 0.6 ± 0.05 
 DTT (4)   2.2 ± 0.2 × 10−4 0.4 ± 0.07 9.9 ± 0.1 × 10−4 0.6 ± 0.07 

For each agonist/receptor/treatment combination, mean open durations and relative areas from a fit of the sum of exponentials were determined. Values listed are the mean ± SD for the indicated number of patches.

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