Table 1.
Mean open durations for different agonists after oxidation and reduction of αD97C/αY127C receptors
Agonist/receptor (number of transfections)Treatment (number of patches)mOpen1Area1mOpen2Area2
ACh/αD97C/αY127C (8) Control (4) 2.3 ± 0.3 × 10−4 0.4 ± 0.08 8.0 ± 0.8 × 10−4 0.7 ± 0.10 
 H2O2 (12) 2.0 ± 0.1 × 10−4 1.0 ± 0.03   
 DTT (8) 3.0 ± 0.1 × 10−4 0.6 ± 0.04 8.5 ± 0.1 × 10−4 0.4 ± 0.06 
ACh/wild type (3) Control (4) 1.9 ± 0.1 × 10−5 0.2 ± 0.05 1.2 ± 0.1 × 10−3 0.8 ± 0.08 
 H2O2 (8) 2.0 ± 0.2 × 10−5 0.1 ± 0.01 1.3 ± 0.1 × 10−3 0.9 ± 0.09 
 DTT (6) 1.8 ± 0.1 × 10−5 0.2 ± 0.04 9.4 ± 0.7 × 10−4 0.7 ± 0.06 
SubCho/αD97C/αY127C (5) Control (6)   6.5 ± 0.5 × 10−4 1.0 ± 0.02 
 H2O2 (12) 6.9 ± 0.1 × 10−5 0.2 ± 0.04 1.8 ± 0.5 × 10−4 0.8 ± 0.06 
 DTT (8)   5.4 ± 0.6 × 10−4 1.0 ± 0.02 
SubCho/wild type (3) Control (4) 2.6 ± 0.14 × 10−5 0.3 ± 0.05 2.9 ± 0.2 × 10−4 0.7 ± 0.08 
 H2O2 (4) 1.9 ± 0.11 × 10−5 0.4 ± 0.06 2.8 ± 0.1 × 10−4 0.6 ± 0.09 
 DTT (4) 2.1 ± 0.14 × 10−5 0.3 ± 0.05 2.7 ± 0.1 × 10−4 0.7 ± 0.10 
CCh/αD97C/αY127C (3) Control (4) 7.3 ± 0.2 × 10−5 0.8 ± 0.07 1.8 ± 0.8 × 10−4 0.3 ± 0.07 
 H2O2 (6) 6.0 ± 0.9 × 10−5 0.7 ± 0.06 2.8 ± 0.1 × 10−4 0.3 ± 0.04 
 DTT (8) 4.9 ± 0.5 × 10−5 0.7 ± 0.06 2.3 ± 0.2 × 10−4 0.3 ± 0.05 
CCh/wild type (3) Control (4)   3.2 ± 0.3 × 10−4 1.0 ± 0.03 
 H2O2 (6)   3.1 ± 0.3 × 10−4 1.0 ± 0.02 
 DTT (4)   2.9 ± 0.3 × 10−4 1.0 ± 0.03 
PIP/αD97C/αY127C (3) Control (4)   2.1 ± 0.1 × 10−4 1.0 ± 0.03 
 H2O2 (6)   2.1 ± 0.5 × 10−4 1.0 ± 0.02 
 DTT (6)   2.2 ± 0.7 × 10−4 1.0 ± 0.05 
PIP/wild type (3) Control (4)   2.5 ± 0.2 × 10−4 1.0 ± 0.04 
 H2O2 (4)   2.9 ± 0.2 × 10−4 1.0 ± 0.03 
 DTT (4)   2.9 ± 0.2 × 10−4 1.0 ± 0.03 
TMA/αD97C/αY127C (3) Control (4)   3.8 ± 1.0 × 10−4 1.0 ± 0.02 
 H2O2 (6)   4.1 ± 0.3 × 10−4 1.0 ± 0.03 
 DTT (4)   3.8 ± 0.5 × 10−4 1.0 ± 0.03 
TMA/wild type (3) Control (4)   3.9 ± 0.4 × 10−4 1.0 ± 0.03 
 H2O2 (4)   4.1 ± 0.2 × 10−4 1.0 ± 0.03 
 DTT (4)   3.9 ± 0.3 × 10−4 1.0 ± 0.05 
Cho/αD97C/αY127C (4) Control (4)   2.1 ± 0.2 × 10−4 1.0 ± 0.03 
 H2O2 (8)   2.0 ± 0.3 × 10−4 1.0 ± 0.03 
 DTT (6)   2.2 ± 0.7 × 10−4 1.0 ± 0.04 
Agonist/receptor (number of transfections)Treatment (number of patches)mOpen1Area1mOpen2Area2
ACh/αD97C/αY127C (8) Control (4) 2.3 ± 0.3 × 10−4 0.4 ± 0.08 8.0 ± 0.8 × 10−4 0.7 ± 0.10 
 H2O2 (12) 2.0 ± 0.1 × 10−4 1.0 ± 0.03   
 DTT (8) 3.0 ± 0.1 × 10−4 0.6 ± 0.04 8.5 ± 0.1 × 10−4 0.4 ± 0.06 
ACh/wild type (3) Control (4) 1.9 ± 0.1 × 10−5 0.2 ± 0.05 1.2 ± 0.1 × 10−3 0.8 ± 0.08 
 H2O2 (8) 2.0 ± 0.2 × 10−5 0.1 ± 0.01 1.3 ± 0.1 × 10−3 0.9 ± 0.09 
 DTT (6) 1.8 ± 0.1 × 10−5 0.2 ± 0.04 9.4 ± 0.7 × 10−4 0.7 ± 0.06 
SubCho/αD97C/αY127C (5) Control (6)   6.5 ± 0.5 × 10−4 1.0 ± 0.02 
 H2O2 (12) 6.9 ± 0.1 × 10−5 0.2 ± 0.04 1.8 ± 0.5 × 10−4 0.8 ± 0.06 
 DTT (8)   5.4 ± 0.6 × 10−4 1.0 ± 0.02 
SubCho/wild type (3) Control (4) 2.6 ± 0.14 × 10−5 0.3 ± 0.05 2.9 ± 0.2 × 10−4 0.7 ± 0.08 
 H2O2 (4) 1.9 ± 0.11 × 10−5 0.4 ± 0.06 2.8 ± 0.1 × 10−4 0.6 ± 0.09 
 DTT (4) 2.1 ± 0.14 × 10−5 0.3 ± 0.05 2.7 ± 0.1 × 10−4 0.7 ± 0.10 
CCh/αD97C/αY127C (3) Control (4) 7.3 ± 0.2 × 10−5 0.8 ± 0.07 1.8 ± 0.8 × 10−4 0.3 ± 0.07 
 H2O2 (6) 6.0 ± 0.9 × 10−5 0.7 ± 0.06 2.8 ± 0.1 × 10−4 0.3 ± 0.04 
 DTT (8) 4.9 ± 0.5 × 10−5 0.7 ± 0.06 2.3 ± 0.2 × 10−4 0.3 ± 0.05 
CCh/wild type (3) Control (4)   3.2 ± 0.3 × 10−4 1.0 ± 0.03 
 H2O2 (6)   3.1 ± 0.3 × 10−4 1.0 ± 0.02 
 DTT (4)   2.9 ± 0.3 × 10−4 1.0 ± 0.03 
PIP/αD97C/αY127C (3) Control (4)   2.1 ± 0.1 × 10−4 1.0 ± 0.03 
 H2O2 (6)   2.1 ± 0.5 × 10−4 1.0 ± 0.02 
 DTT (6)   2.2 ± 0.7 × 10−4 1.0 ± 0.05 
PIP/wild type (3) Control (4)   2.5 ± 0.2 × 10−4 1.0 ± 0.04 
 H2O2 (4)   2.9 ± 0.2 × 10−4 1.0 ± 0.03 
 DTT (4)   2.9 ± 0.2 × 10−4 1.0 ± 0.03 
TMA/αD97C/αY127C (3) Control (4)   3.8 ± 1.0 × 10−4 1.0 ± 0.02 
 H2O2 (6)   4.1 ± 0.3 × 10−4 1.0 ± 0.03 
 DTT (4)   3.8 ± 0.5 × 10−4 1.0 ± 0.03 
TMA/wild type (3) Control (4)   3.9 ± 0.4 × 10−4 1.0 ± 0.03 
 H2O2 (4)   4.1 ± 0.2 × 10−4 1.0 ± 0.03 
 DTT (4)   3.9 ± 0.3 × 10−4 1.0 ± 0.05 
Cho/αD97C/αY127C (4) Control (4)   2.1 ± 0.2 × 10−4 1.0 ± 0.03 
 H2O2 (8)   2.0 ± 0.3 × 10−4 1.0 ± 0.03 
 DTT (6)   2.2 ± 0.7 × 10−4 1.0 ± 0.04 

For each agonist/receptor/treatment combination, mean open durations and relative areas from a fit of the sum of exponentials were determined. Values listed are the mean ± SD for the indicated number of patches.

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