Table 1. Constructs used in the... | Rockefeller University Press

Table 1.

Constructs used in the experiments and steady-state parameters

Construct	V_h,cont	zδ_cont	V_h,cAMP	zδ_cAMP	V_h,contV_h,cAMP
Nonconcatenated subunits
w₄	−116.5 ± 1.8	6.57 ± 0.40	−94.8 ± 1.5	6.42 ± 0.48	21.6 ± 1.1
m₄	−120.7 ± 1.6	6.64 ± 0.44	−121.5 ± 1.2	6.67 ± 0.42	0.04 ± 0.7
Dimeric concatemers
ww₂	−125.3 ± 2.0	6.36 ± 0.50	−104.5 ± 2.2	7.56 ± 0.30	20.9 ± 1.4
mm₂	−130.9 ± 0.6	6.11 ± 0.21	−131.8 ± 1.7	6.26 ± 0.17	−0.94 ± 1.3
wm₂	−115.3 ± 1.2	7.09 ± 0.32	−105.6 ± 1.0	6.42 ± 0.32	9.0 ± 0.7
Tetrameric concatemers
wwww	−123.9 ± 2.3	6.41 ± 0.26	−103.9 ± 3.0	6.43 ± 0.26	21.1 ± 1.1
wwwm	−116.7 ± 1.0	6.00 ± 0.31	−102.0 ± 0.8	6.64 ± 0.26	14.8 ± 1.1
wmwm	−120.2 ± 1.2	6.07 ± 0.39	−111.7 ± 1.6	6.09 ± 0.34	9.7 ± 1.6
mwmw	−119.7 ± 0.8	9.03 ± 0.81	−109.9 ± 0.8	8.79 ± 0.50	9.8 ± 0.6
mmww	−130.9 ± 0.9	6.20 ± 0.20	−121.5 ± 0.9	5.25 ± 0.17	9.4 ± 0.5
wwmm	−127.6 ± 1.2	6.02 ± 0.69	−119.3 ± 1.2	5.12 ± 0.42	10.3 ± 1.7
mmmw	−126.6 ± 0.9	6.44 ± 0.46	−122.5 ± 1.1	6.30 ± 0.37	4.8 ± 1.1
mmmm	−126.1 ± 1.5	6.27 ± 0.44	−128.8 ± 1.4	6.38 ± 0.75	−2.2 ± 0.8

Construct	V_h,cont	zδ_cont	V_h,cAMP	zδ_cAMP	V_h,contV_h,cAMP
Nonconcatenated subunits
w₄	−116.5 ± 1.8	6.57 ± 0.40	−94.8 ± 1.5	6.42 ± 0.48	21.6 ± 1.1
m₄	−120.7 ± 1.6	6.64 ± 0.44	−121.5 ± 1.2	6.67 ± 0.42	0.04 ± 0.7
Dimeric concatemers
ww₂	−125.3 ± 2.0	6.36 ± 0.50	−104.5 ± 2.2	7.56 ± 0.30	20.9 ± 1.4
mm₂	−130.9 ± 0.6	6.11 ± 0.21	−131.8 ± 1.7	6.26 ± 0.17	−0.94 ± 1.3
wm₂	−115.3 ± 1.2	7.09 ± 0.32	−105.6 ± 1.0	6.42 ± 0.32	9.0 ± 0.7
Tetrameric concatemers
wwww	−123.9 ± 2.3	6.41 ± 0.26	−103.9 ± 3.0	6.43 ± 0.26	21.1 ± 1.1
wwwm	−116.7 ± 1.0	6.00 ± 0.31	−102.0 ± 0.8	6.64 ± 0.26	14.8 ± 1.1
wmwm	−120.2 ± 1.2	6.07 ± 0.39	−111.7 ± 1.6	6.09 ± 0.34	9.7 ± 1.6
mwmw	−119.7 ± 0.8	9.03 ± 0.81	−109.9 ± 0.8	8.79 ± 0.50	9.8 ± 0.6
mmww	−130.9 ± 0.9	6.20 ± 0.20	−121.5 ± 0.9	5.25 ± 0.17	9.4 ± 0.5
wwmm	−127.6 ± 1.2	6.02 ± 0.69	−119.3 ± 1.2	5.12 ± 0.42	10.3 ± 1.7
mmmw	−126.6 ± 0.9	6.44 ± 0.46	−122.5 ± 1.1	6.30 ± 0.37	4.8 ± 1.1
mmmm	−126.1 ± 1.5	6.27 ± 0.44	−128.8 ± 1.4	6.38 ± 0.75	−2.2 ± 0.8

The sequence of either two or four w or m denotes a concatemer with the respective subunit arrangement reading from N terminus to C terminus. The data points were fitted with the Boltzmann function (Eq. 1), yielding the effective gating charge zδ and the voltage of half-maximum activation V_h. The errors are given as SEM. w, wild-type subunit; m, subunit carrying the mutation R591E; w₄ and m₄, channels formed from nonligated subunits.

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