Table 1. Numerical values of the... | Rockefeller University Press

Table 1.

Numerical values of the interaction between SthK and cyclic nucleotides

Cyclic nucleotide assay	K_d (µM)	SEM
Direct binding assay
fcAMP	0.6	0.1
fcGMP	2.7	0.5
Competition assay
cAMP	36	12
cGMP	28	9
Activation of SthK by cAMP	EC₅₀ (µM)	SEM
Bilayer recordings	17	0.8
Stopped flow	107	8
Inhibition of SthK by cGMP	K_i (µM)	SEM
Bilayer recordings	4	0.5
Stopped flow	6	0.7

Cyclic nucleotide assay	K_d (µM)	SEM
Direct binding assay
fcAMP	0.6	0.1
fcGMP	2.7	0.5
Competition assay
cAMP	36	12
cGMP	28	9
Activation of SthK by cAMP	EC₅₀ (µM)	SEM
Bilayer recordings	17	0.8
Stopped flow	107	8
Inhibition of SthK by cGMP	K_i (µM)	SEM
Bilayer recordings	4	0.5
Stopped flow	6	0.7

Table 1 provides the numerical values obtained from the binding and activation studies of SthK. K_i values for cGMP inhibition were calculated according to Eq. 12. The listed values are correlated with the data shown in Figs. 2, 3, 4, and 5 and represent means ± SEM for at least three independent measurements.

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