Table 1. Kinetic effects of halothane on... | Rockefeller University Press

Table 1.

Kinetic effects of halothane on action potential–induced Ca²⁺ release

Halothane (%)	τ₁ (ms)	P-value	A₂	P-value	τ₂ (ms)	P-value	Baseline	P-value	Peak	P-value	n
Free [Ca²⁺] (µM) WT
0	0.552 ± 0.017		0.376 ± 0.012		15.58 ± 0.95		0.0203 ± 0.0016		6.74 ± 0.36		14
3	0.652 ± 0.041	0.0373^{^a}	0.371 ± 0.020	0.84	22.16 ± 1.80	0.00447^{^b}	0.0284 ± 0.0013	0.000548^{^c}	6.51 ± 0.40	0.673	13
Free [Ca²⁺] (µM) YS
0	0.693 ± 0.029		0.327 ± 0.016		18.17 ± 1.14		0.0433 ± 0.0046		4.81 ± 0.24		20
3	0.916 ± 0.080	0.0162^{^a}	0.338 ± 0.016	0.6	32.30 ± 3.42	0.000881^{^c}	0.0834 ± 0.0144	0.0156^{^a}	3.32 ± 0.24	0.000103^{^c}	17
Ca²⁺ Flux (mM/s) WT
0	0.649 ± 0.030		0.143 ± 0.012		12.71 ± 0.88		0.244 ± 0.019		343.71 ± 20.24		14
3	0.688 ± 0.030	0.363	0.153 ± 0.012	0.553	18.94 ± 1.65	0.00363^{^b}	0.340 ± 0.015	0.000548^{^c}	322.30 ± 17.29	0.429	13
Ca²⁺ Flux (mM/s) YS
0	0.731 ± 0.030		0.115 ± 0.008		15.05 ± 1.34		0.519 ± 0.056		253.68 ± 13.01		20
3	0.855 ± 0.053	0.0526	0.119 ± 0.008	0.697	28.86 ± 3.86	0.003^{^b}	1.000 ± 0.172	0.0156^{^a}	163.76 ± 12.60	0.0000179^{^c}	17

Halothane (%)	τ₁ (ms)	P-value	A₂	P-value	τ₂ (ms)	P-value	Baseline	P-value	Peak	P-value	n
Free [Ca²⁺] (µM) WT
0	0.552 ± 0.017		0.376 ± 0.012		15.58 ± 0.95		0.0203 ± 0.0016		6.74 ± 0.36		14
3	0.652 ± 0.041	0.0373^{^a}	0.371 ± 0.020	0.84	22.16 ± 1.80	0.00447^{^b}	0.0284 ± 0.0013	0.000548^{^c}	6.51 ± 0.40	0.673	13
Free [Ca²⁺] (µM) YS
0	0.693 ± 0.029		0.327 ± 0.016		18.17 ± 1.14		0.0433 ± 0.0046		4.81 ± 0.24		20
3	0.916 ± 0.080	0.0162^{^a}	0.338 ± 0.016	0.6	32.30 ± 3.42	0.000881^{^c}	0.0834 ± 0.0144	0.0156^{^a}	3.32 ± 0.24	0.000103^{^c}	17
Ca²⁺ Flux (mM/s) WT
0	0.649 ± 0.030		0.143 ± 0.012		12.71 ± 0.88		0.244 ± 0.019		343.71 ± 20.24		14
3	0.688 ± 0.030	0.363	0.153 ± 0.012	0.553	18.94 ± 1.65	0.00363^{^b}	0.340 ± 0.015	0.000548^{^c}	322.30 ± 17.29	0.429	13
Ca²⁺ Flux (mM/s) YS
0	0.731 ± 0.030		0.115 ± 0.008		15.05 ± 1.34		0.519 ± 0.056		253.68 ± 13.01		20
3	0.855 ± 0.053	0.0526	0.119 ± 0.008	0.697	28.86 ± 3.86	0.003^{^b}	1.000 ± 0.172	0.0156^{^a}	163.76 ± 12.60	0.0000179^{^c}	17

The alterations in single action potential–induced responses demonstrated in Fig. 2 were quantified by nonlinear regression analysis. The relaxation phases of baseline-subtracted and peak-normalized free [Ca²⁺] and Ca²⁺ release flux traces were fitted by a dual exponential function: f(t) = (1 − A₂) · exp[−(t − t₀)/τ₁] + A₂ · exp[−(t − t₀)/τ₂]. The fit interval was 100 ms and started when traces had decreased by 5% of their peak value. Errors, SEM; n, number of experiments. P-values refer to changes caused by halothane.

P < 0.05.

P < 0.01.

P < 0.001.

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