Table 2.
Binding of thiamine to PnuTSw mutants determined by isothermal titration calorimetry
PnuTSw mutantDissociation constant Kd
 µM 
Y159F 23 ± 2 
Y162F 26 ± 3 
Y188A 21 ± 2 
Y77A 17 ± 2 
D166A No binding 
W199A 12 ± 1 
W88A 13 ± 2 
PnuTSw mutantDissociation constant Kd
 µM 
Y159F 23 ± 2 
Y162F 26 ± 3 
Y188A 21 ± 2 
Y77A 17 ± 2 
D166A No binding 
W199A 12 ± 1 
W88A 13 ± 2 

The error represents the SD obtained from three independent measurements.

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