Table 1. Binding of thiamine and... | Rockefeller University Press

Table 1.

Binding of thiamine and analogues to PnuT_Sw determined by isothermal titration calorimetry

Substrate	Dissociation constant K_d	ΔH	ΔS
	µM	kJ/mol	J/mol/K
Thiamine	12 ± 5	−27 ± 22	5 ± 71
Pyrithiamine	4.4 ± 1	−18 ± 13	41 ± 43
TMP	No binding
TPP	No binding
Oxythiamine	1.3 * 10² ± 25	−18 ± 6	14 ± 19

Substrate	Dissociation constant K_d	ΔH	ΔS
	µM	kJ/mol	J/mol/K
Thiamine	12 ± 5	−27 ± 22	5 ± 71
Pyrithiamine	4.4 ± 1	−18 ± 13	41 ± 43
TMP	No binding
TPP	No binding
Oxythiamine	1.3 * 10² ± 25	−18 ± 6	14 ± 19

The error represents the SD obtained from three (pyrithiamine, oxythiamine) or five (thiamine) independent measurements.

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