| Monomer/dimer distributions . | ΔG°WT . | ΔG°W . |
|---|---|---|
| kcal/mol | kcal/mol | |
| PM: 400-nm EPL Walden (KD = 1 × 10100) | −11.4 ± 0.2 (0.84) | −9.5 ± 0.2 (0.85) |
| PD: 400-nm EPL Walden (KD = 1 × 10−100) | ||
| PM: 400-nm 2:1 POPE/POPG (KD = 1 × 10100) | −11.3 ± 0.2 (0.70) | −8.4 ± 0.2 (0.45) |
| PD: 400-nm 2:1 POPE/POPG (KD = 1 × 10−100) | ||
| PM: WW | −10.7 ± 0.2 (0.75) | −8.5 ± 0.2 (0.80) |
| PD: WT + glutaraldehyde | ||
| PM: WW | −10.9 ± 0.1 (0.90) | −8.8 ± 0.2 (0.83) |
| PD: R230C/L249C |
| Monomer/dimer distributions . | ΔG°WT . | ΔG°W . |
|---|---|---|
| kcal/mol | kcal/mol | |
| PM: 400-nm EPL Walden (KD = 1 × 10100) | −11.4 ± 0.2 (0.84) | −9.5 ± 0.2 (0.85) |
| PD: 400-nm EPL Walden (KD = 1 × 10−100) | ||
| PM: 400-nm 2:1 POPE/POPG (KD = 1 × 10100) | −11.3 ± 0.2 (0.70) | −8.4 ± 0.2 (0.45) |
| PD: 400-nm 2:1 POPE/POPG (KD = 1 × 10−100) | ||
| PM: WW | −10.7 ± 0.2 (0.75) | −8.5 ± 0.2 (0.80) |
| PD: WT + glutaraldehyde | ||
| PM: WW | −10.9 ± 0.1 (0.90) | −8.8 ± 0.2 (0.83) |
| PD: R230C/L249C |
Free energies are calculated as ΔG° = −RT ln(Keq χ°), where χ° is the mole fraction standard state of 1 subunit/lipid, R is the gas constant 1.99 × 10−3 kcal mole−1 K−1, and T is 298 K (25°C), where Keq (lipids/subunit) is determined by fitting to the equilibrium dimerization isotherm. Data represent best-fit ± error of fit (R2). KD, dissociation constant (subunit/lipid) equal to 1/Keq; PM, nonreactive monomer distribution; PD, nonreactive dimer distribution.