Table 1.
Isolation protocol comparison
ProcedureProtocol 1Protocol 2Protocol 3
Buffer delivery Constant flow by minipump Gravity-induced flow Constant pressure monitored and adjusted by minipump 
Cannulation Excision, then cannulation In-chest cannulation In-chest cannulation 
Buffer contents NaCl, KCl, NaH2PO4, NaHCO3, MgSO4, glucose, BDM, taurine NaCl, KCl, MgCl2, HEPES, glucose, taurine, Na2HPO4 NaCl, KCl, MgSO4, Na2HPO4, KH2PO4, NaHCO3, KHCO3, HEPES, glucose 
Isolation initial survivorship rate 49–54% rod shaped 73–83% rod shaped 80–90% rod shaped 
Basal CFP/YFP ratio (a.u.) 0.9925–1.0051 0.9957–1.0012 0.9802–0.9972 
Normalized 40 h AKAR3 FRET Ratio change (a.u.) 1.245–1.2514 1.236–1.2416 1.267–1.2692 
ProcedureProtocol 1Protocol 2Protocol 3
Buffer delivery Constant flow by minipump Gravity-induced flow Constant pressure monitored and adjusted by minipump 
Cannulation Excision, then cannulation In-chest cannulation In-chest cannulation 
Buffer contents NaCl, KCl, NaH2PO4, NaHCO3, MgSO4, glucose, BDM, taurine NaCl, KCl, MgCl2, HEPES, glucose, taurine, Na2HPO4 NaCl, KCl, MgSO4, Na2HPO4, KH2PO4, NaHCO3, KHCO3, HEPES, glucose 
Isolation initial survivorship rate 49–54% rod shaped 73–83% rod shaped 80–90% rod shaped 
Basal CFP/YFP ratio (a.u.) 0.9925–1.0051 0.9957–1.0012 0.9802–0.9972 
Normalized 40 h AKAR3 FRET Ratio change (a.u.) 1.245–1.2514 1.236–1.2416 1.267–1.2692 
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